Pros And Cons Of Genetic Transformation

Throughout the experiment, many aspects of how it was conducted were weak and lacking a method and plan. One weakness in the experimental plan was the lack of selecting which lab member would do what portion of the experiment prior to the experiment starting. Therefore, some lab group members were conducting sections of the experiment that might have been unclear to the individual. Another weakness in the experiment was the collection of the results. The lab group waited a week before collecting results and could have lost some feeling of ‘freshness’ with the experiment and not recorded the results in great detail. Also during data collection, there was a lack of communication between group members in regards to the undesired results which

The addition of other samples would have broadened the results and allowed a hypothesis to be more overarching about genetic transformation as a whole. One additional plate that could have been added would have been a Sample 5 whose environment contained +pGLO and the Luria Burtani broth. Sample 5 would have been an interesting sample to experiment with because it would not have contained the ampicillin resistance gene, and therefore would not have grown. This is because the ampicillin resistance gene tests which cells have taken up the plasmid when they’re plated. If the ampicillin resistance gene is not present, all the cells will be considered “not transformed” and will not survive, let alone grow. A secondary sample that could have been tested, Sample 6, could contain an environment with -pGLO, Luria Bertani broth, and arabinose. This sample’s results should further illustrate the results of the lab, as due to a lack of +pGLO the bacteria should not grow, even with the arabinose sugar as a food source. Furthermore, due to a lacking of ampicillin resistance, again the bacteria should not show any growth as the cells will be considered “not transformed” and will not survive the plating. The addition of more samples would help broaden the understanding of genetic transformation and further illustrate the main point of the