Bacterial culture is a perfect way to study the growth rate. This method is not specified for a matter, it is a necessary part of microbiological studies. The growth of bacterial cells is affected by different factors such as temperature, waste, pH and nutrients resources. Since the study is related to numbers and amount of substances, mathematical methods are needed to know the quantity of bacteria in the experiment hence, exponential. In addition, growth rate is proportional to the number of cells. As the nutrients supplies increase the amount of bacteria increase. However, when the nutrients are less than the normal state, the cell division will decrease hence, the bacterial cell might be negligible, as the rate of the death will exceed the rate of growth cells. According to Henrici, the size of the cells is different in the growth phases that bacteria goes throw it, log phase, lag phase, stationary and death. Also Monod has study about growth rate that depend on nutrients.
INTRODUCTION:
In 1674 there was a scientist who is called Anton Van Leeuwnhoek, he was the first human who discovered microscopic living organisms called bacteria. This is the beginning of bacteria culture in which experiments are done on it. There are some factors effects on bacteria growing like temperature, PH, wastes, nutrients resources. In this report we will study the dependence of growth rates on nutrients. Bacteria growth is finite and it is known as the increment of size and cell mass. A
The Task:
We started learning about exponents at the beginning of the nuclear culture unit. We learned about positive, negative, fractional, and zero exponents and their effects on the graph. Exponents make a graph exponential, meaning that the graph will either drastically increase rapidly or drastically decrease rapidly. We used our knowledge of exponential growth and decay to understand topics such as interest, population growth and decay, disease growth or decay, radioactive decay, and bacterial growth or decay; after this unit we were able to understand these topics and graph them. The most beneficial concept in this unit was understanding how interest grows and decays.
Exercise 14: Unknown Identification Lab Report
The purpose of the study was to identify the unknown bacterium using various biochemical tests in addition to using scientific methods in determining the outcome of the hypothesis. Each biochemical test will help determine the bacteria based on specific characteristics of each organism. I was giving unknown number 232. The first procedure that needed to be done after obtaining unknown bacterial mixture was to isolate the two bacteria in a pure culture using the streak plate method described in Microbiology Laboratory Manual Eight Edition. The material used was trypticase soy agar (TSA) plate, nutrient plate, starch agar, hydrogen peroxide, iodine reagent and microscope.
Bacteria are living things that have only one cell. Under a microscope, they look like balls, rods, or spirals. Many are helpful. Some bacteria help to digest food, destroy disease-causing cells, and give the body needed vitamins. Bacteria are also used in making healthy foods like yogurt.
Figure 3. Testing of transformed and mutant bacteria on minimal medium
Growth was observed on the Transformed (Trsf) section and not on the Mutant (Mut)
I expect to learn the biochemical differences in bacteria from this lab. Also, how to identify different species of bacteria.
Material & Methods
For the first day of the practical, an unknown specimen was provided
These microorganisms are used to teach us how multicellular organisms came to be and how they can survive today. These small, microscopic organisms are so unique that the identification of them is paramount in the advancements of science. Knowing the chemical makeup, the shape, and the biochemical processes is important in identifying these organisms to understand how they survive and where.
A number of tests can be ran on an unknown bacteria to determine their ideal
The purpose of this experiment was to insert the plasmid glow green into the bacteria with a gene of interest to produce the protein that make the bacteria glow green along with the presence of arabinose and the presence of ampicillin. Many scientists are experimenting different kind of genes that can inserted into the organism for survival. The technique of transformation was used in this experiment to give the organism a new trait that they did not possess in their life. In this experiment, the bacteria were added to four plates with certain conditions such as the existence of plasmid, ampicillin, and arabinose to see whether the bacteria grow and glow green. The results showed that the LB/amp/araC +pGLO produce a lot of colony and most
Meanwhile, on our LB/amp plus DNA petri dish we had a lawn of bacteria and the color was almost clear. Our results for this petri dish mean that the bacteria was not killed by the ampicillin. Next, in the LB/amp minus DNA petri dish we observed very minimal amounts of growth. These results mean that we correctly followed the procedures because this petri dish was not supposed to have growth due to the ampicillin. Our last petri dish was minus DNA and lysogeny broth, in this petri dish we saw 7 colonies of bacteria and there were a light yellow color.
coli bacteria new traits. The pGLO plasmid that is being transformed into these cells contains genes that can give colonies of bacteria the ability of antibiotic resistance and a green fluorescent glow. Four different models were prepared and plated on multiple agar plate. After the bacteria was grown for three days in an incubator at 37°C; observations were made and recorded (Table 1). All of the plates were looked at for the amount of colonies grown, if growth was present, and if the colonies gained the ability to glow green.
INTRODUCTION:
In this experiment I was testing for antimicrobial sensitivity of Staphylococcus epidermidis by using the Kirby-Bauer Diffusion test. The three antibiotics utilized in this lab were: gentamicin, novobiocin, and penicillin. I determined the effectiveness of the antibiotic by observing and measuring the zone of inhibition for each antibiotic.
The B. Vulgaris samples were approximately 1cm3. They were kept the same size to ensure accurate results. A control test was conducted in distilled water to obtain a result to compare. The ethanol treatments were 40% and 70%. To prepare the solutions a 70% ethanol solution was used to make 40%.
To prepare the solutions a 70% ethanol solution was used to make 40%. This was calculated using the C1V1=C2V2 formula. A photo spectrometer was used to measure, in arbitrary units, the change in membrane permeability of the B. Vulgaris cells. To begin, the B. Vulgaris samples were put into vials containing the distilled water, 40% and 70% Ethanol solutions. As soon as the B. Vulgaris samples were added to the vials a time zero sample was taken from the vials.
Escherichia Coli 0157: H7
This paper will specialize on a specific type of bacterial foodborne illness caused by the bacteria Escherichia Coli. E. coli was discovered by Theodore von Escherich in 1885. E.coli is a natural found bacteria that lies throughout the intestinal tract of warm blooded animals and comes in many forms only one of which is deadly. This form is E. coli 0157:H7 which can be caused by direct exposure to fecal matter to kill this rouge
The media used in this experiment was Trypticase nitrate broth. The reagents used (A and B) were sulfanilic acid and alpha-naphthylamine (respectively). Using aseptic technique, the bacterium (16A and 16B) were inoculated into labeled broth test tubes. The tubes were incubated for 48 hours at 37 degrees Celsius. When the incubation was complete 5 drops of reagent A and 5 drops of reagent B were added to each of the broths.
