Hunting nightmare bacteria Answer the following questions Case of Addy (the girl from Arizona ) 1- Based on the pediatrician observations what was Addy’s diagnosis at the Pediatric Hospital intensive care unit ? She had got infected by staff or positive bacteria called Methicillin-resistant Staphylococcus aureus (MRSA). MRSA is a community associated bacteria that infects kids when they are playing in playing ground and getting scabs on their knee. They spread through that wound and it has very high resistance to antibiotics.
Exercise 14: Unknown Identification Lab Report The purpose of the study was to identify the unknown bacterium using various biochemical tests in addition to using scientific methods in determining the outcome of the hypothesis. Each biochemical test will help determine the bacteria based on specific characteristics of each organism. I was giving unknown number 232. The first procedure that needed to be done after obtaining unknown bacterial mixture was to isolate the two bacteria in a pure culture using the streak plate method described in Microbiology Laboratory Manual Eight Edition. The material used was trypticase soy agar (TSA) plate, nutrient plate, starch agar, hydrogen peroxide, iodine reagent and microscope.
Bacteria are living things that have only one cell. Under a microscope, they look like balls, rods, or spirals. Many are helpful. Some bacteria help to digest food, destroy disease-causing cells, and give the body needed vitamins. Bacteria are also used in making healthy foods like yogurt.
Differential media allows for the differentiation between two similar micro-organisms through how the bacteria may handle certain compounds found in the media or the different reactions that may take place when the bacteria is exposed to the medium (3). Selective media on the other hand allow only certain microbes to grow. This is due to the plate containing a limited amount of nutrients, compounds and chemicals that will deter the growth of certain bacteria (3). Dyes, antimicrobial substances, salts, certain growth inhibitors and, antibiotics are also found on this type of medium (3). The differential and selective media mentioned in this lab are as follows:
The unknown bacteria was then tested on multiple selective and differential media. Growth was present on the MacConkey Agar and the colonies were the same color as the plate, which told me my bacteria was gram negative and did not ferment lactose. There was no growth on the Mannitol Salt Agar, and this told me the unknown was not salt tolerant and did not
These microorganisms are used to teach us how multicellular organisms came to be and how they can survive today. These small, microscopic organisms are so unique that the identification of them is paramount in the advancements of science. Knowing the chemical makeup, the shape, and the biochemical processes is important in identifying these organisms to understand how they survive and where. A number of tests can be ran on an unknown bacteria to determine their ideal
In the book Missing Microbes, the author, Dr. Martin J. Blaser discusses different types where the mysterious microbes are to be found. Dr. Martin also discusses his hypothesis in which talks about how over use of antibiotics has permanently changed the microbiome that humans live in, causing an increase in more modern diseases. The way Blaser lays the book is more like a journey; he traces his footsteps, and has the readers following the lead anxiously waiting on what he will inform them. There are a lot of doors in Science. Dr. Blaser chose to enter the door where facts and stories are to be learned everyday, in which there is no end, making that the beauty of science.
Transformation was successful in the plates where the bacteria consumed the pGLO plasmid. In the first plate that the bacterium was plated on it included the LB broth and of ampicillin antibiotic (amp), 2 colonies were present. The second plate of bacteria was grown with the presence of LB broth, ampicillin, arabinose sugar (ara), and 22 colonies were observed. But a green fluorescent glow of the colonies was only present in plate 2. Plates 3 and 4 were the control plates.
Being able to identify unknown microbes from systematic testing is what makes the field of microbiology so important, especially in infectious disease control. Using the testing procedure laid out by the microbiology field we are able to identify unknown bacteria present in our everyday lives, and along the way learn a lot about their characteristics that separate them from other types of bacteria. Being able to do this is vital in order for us to understand why microbes are present in certain places, how they are able to grow and what restricts their growth, that way they can be combatted if necessary. These techniques for determining unknowns are also important for isolating and testing infectious disease microbes in order to prevent spreading. Another important aspect of being able to identify unknown microbes is the
Antibiotics the Good and the Bad Is society aware that they could be facing a major problem created by themselves if antibiotics are not used correctly? Resistance against antibiotics has just recently become a problem. Farmers use antibiotics by giving them to their livestock, which does have many benefits, but also has a downside after being harvested. Overusing antibiotics is thought to lead to resistant strains of bacteria that no antibiotic can cure. Some believe that antibiotics should be used in food animals because it treats illnesses and promotes growth, while others believe antibiotics should not be used because the resistant strains could end up affecting humans through their meat.
You see, it was the Java based image processing program Imagej, that made it possible for me to complete my research on time for the International Science and Engineering Fair (ISEF) in 2016. I had six days to measure the bacterial colony areas of the control and variable groups of Gram positive and negative bacteria. Manual measuring was not an option. I therefore googled “image processing programs to measure bacterial colony area” and a research article in PLOS, High-Throughput Method for Automated Colony and Cell Counting by Digital Image Analysis Based on Edge Detection, described the development of ImageJ, exactly what I was looking for.
Biological warfare has been used “…since the onset of human civilization…” (Kostadinov and Galabova, 2010, p. 295). If populations were able to use biological agents back then, it is naïve to think that terrorists will not use them as a weapon of mass destruction. The 2001 Anthrax attacks were the first Anthrax attacks to occur in the U.S. in 25 years (NPR.Org, 2011, p. 2). This attack proves that biological warfare will always be a risk.
Experiment 3: Cell Viability Testing and Counting with Trypan Blue Exclusion Method Anna Coretta R. Santos I. Introduction Cell viability assay plays a crucial role is in the cells of the cell culture. This assay can be used to identify the relationship of cell count from the cell’s behavior (Stoddart 2011). This test help researchers to determine the number of viable and non-viable cells in a cell culture. This assay helps in estimating the cell count of a cell culture and to determine if there is sufficient amount of viable cells in the cell culture that a person will use for future research (Riss et al. 2013).
INTRODUCTION: Quick look at your hands do you see them. (attention) Do I see what you might be asking? Well the millions of bacteria that are currently hanging out on your hands.
This can be tested by simply mixing the serum of suspected individual which contain the antibodies with the antigens of specific bacteria the accumulation of clumps confirms the presence of particular bacterial infection.[2] This test can be performed in various ways including slide agglutination reaction, tube agglutination reaction, indirect agglutination inhibition reactions etc. Another important practical application involves blood group test of
