Bioenergy is one of the sources to reduce the greenhouse gas (GHG) emission and as sources of fuels. Energy can be divided into two types that are renewable and non-renewable. Renewable energy is come from natural sources such as wind, solar and hydroelectric. This energy will not end and does not have limited sources. While non renewable energy is energy that comes from resources that will be ending at the end. Petroleum, coal, and natural gas are example of energy non-renewable energy. So that, as alternative to replace petroleum, the biodiesel from seaweed is used which it comes from sources of renewable energy. Besides that, when use petroleum, it will give many disadvantages such as greenhouse effect, global warning and increase composition of carbon dioxide (CO2) in atmosphere. An atmospheric pollution comes from petroleum diesel when their combustion gives a primer or source for GHG (Sharif et al., 2008). Biodiesel is defined as a fuel made up from monoalkyl esters of long-chain fatty acids derived from bio-oils, usually fatty acid methyl esters (FAME) and can be produced from vegetable oil (Speranza et al. 2015). Macro algae can produce the biodiesel and has a lower lipid compare to the microalgae (Sharif et al., 2008).
In biology, seaweed is macro algae that live in fresh water and sea water that act as a primary producer in the water. It looks plant-like organisms that attach to any substrate such as rock that make photosynthesis to produce glucose as a their food.