Fluorescence Spectrophotometer

1.1 Background Study
In this research study, the ability and suitability of a sensing system based on PVC membrane for determination of volatile organic compounds (VOCs) was investigated. Pyrrole reagent will be immobilized onto the PVC film which functioning as a matrix that can hold the reagent in solid manner. This sensor system is then tested to determine volatile organic compounds (VOCs) such as benzene, toluene, ethylbenzene and xylene (BTEX). The analytes and the reagent will react to each other so that the sensing system can be characterized. In these studies, there are some parameters have to be observed; spectral features, reaction response, limit of detection, pH optimization, reproducibility and regeneration. Volatile organic compounds (VOCs) will be measured by taking gas sample and transporting it to laboratory instruments. So, the instrument that will be use is fluorescence spectrophotometer.

1.2 Pollution of Volatile organic compounds (VOCs) in Environment
There is a growing public concern over industrial impact on the environment. Monitoring environmental quality in a broad sense includes global

Any molecule can go into an electronically excited state when exposed to light of a wavelength (energy level) equal to the energy gap between the ground state and excited state. This is known as molecular absorbance of light. The amount of light absorbed is proportional to the concentration of the absorbing molecule. This connection is described in Lambert-Beers law, where the wavelength dependent absorbance A is described Where A is the absorbance I0 and I the intensity of incoming and transmitted light, ε the molar absortivity expressed in L×mol-1×cm-1, c the concentration in mol×L-1 and l the effective pathway of the sample in cm (Lothian, 1963). Measurement of the absorbance of a sample over a wavelength range results in an absorbance