While the solution dissolved, 50 mL of distilled water was added to a 150 mL beaker and heated on the hot plate. When the solution started to boil 2.65 grams of Na2SiO3*5H2O was added to the beaker with a stir bar and heated to a gentle boil. When both solutions began to boil, the sodium silicate solution was slowly added to the sodium aluminate. The solution was kept at 900C for 60 minutes and stirred with stir bar. After 60 minutes, the zeolite solution was cooled for 5 minutes and for the magnetized zeolite , 0.78 grams of FeCl3 and 0.39 grams of FeSO4*7H2O was added to the flask and stirred until the iron parts dissolved.
Next, a 10 mL beaker is filled with 3 mL of HCl and measure 10 mL of ionized water into a 140 mL beaker. Carefully turn on laboratory burner and start cleaning the Nichrome wire by dipping it into concentrated HCl acid. Hold the Nichrome wire on top of the flame and repeat the step until the wire doesn 't show any color. When the wire is clean, dip the wire again with some of the acid and dip it into the solution with the unknown compound in it. Place the wire back into the flame again and observe the color of the flame.
The compounds tested in included the unknown, Calcium Nitrate, Calcium Chloride, Calcium Carbonate, Sodium Chloride, Potassium Chloride, Magnesium Chloride, and Ammonium Chloride. The next test was the pH test. In this test, the aqueous solutions from the flame tests were used again. A piece of pH paper was dipped into the aqueous solutions, a different piece for each solution. The ensuing coloration of the paper was compared to the pH scale and the
Discussion 1. Zn0 (s)+ Cu2+S6+O42-(aq) →Cu0(s) + Zn2+S6+O42-(aq) Zn0(s) → Zn2+(aq) + 2e- Cu2+(aq) + 2e- → Cu0(s) Zn0(s) + Cu2+(aq) → Zn2+(aq) + Cu0(s) Oxidant (oxidizing agent) is the element which reduces in experiment.
These color changes indicate a chemical change, which show that a reaction had occurred. In the first step when o-vanillin and p-toludine, imine was formed. The color change from green to orange suggests that imine appears as orange colored. In the second step, the addition of sodium borohydride reduced the imine into another derivative, which was yellowish lime color. The solution turned clear when acids and anhydrides was added, which indicated the precipitate were dissolved.
Incubate the tubes on ice for 30min and make sure to push the tubes all the way in. During this time, label 4 agar plates with: +pGLO LB/amp, +pGLO LB/amp/ara, -pGLO LB/amp, and -pGLO LB, the nutrients and ampicillin should be integrated within the agar. Next, one of the most important steps, heat shock, is used to assimilate the plasmid
Then they are shocked with a calcium chloride solution that changes the charge on the cell membrane so that the plasmid DNA may be accepted into the cell. This solution must be chilled so that the cell membrane may heal. After incubating the bacterial cells, they are heat shocked to open the pores in the cell membrane to allow the transformation to occur. After being chilled again in order not to melt the agar, the cells are placed in a medium
Grow E Coli on an agar plate and grow about 3 of them. Take the e coli colony and swab it with a q tip once swabbed move the e coli to another plate by streaking them across another plate. Incubate the E Coli at 37 degrees celsius for 24 hours. Move the E Coli into the fridge to prevent overgrowth of the E Coli. Place C Elegans into the plates with the E Coli and leave for 24 hours.
Fill one planter with 200 ml of Miracle-Gro® Potting Mix, fill the second planter with 200 ml of Miracle-Gro® Seed Starting Potting Mix, fill the third planter with 200 ml of Miracle-Gro® Organic Potting Mix, fill the fourth planter with 200 ml of with Sunbury Christian Academy Soil. Measure .25 grams of seed with a balance, then place seeds one centimeter down into the soil of each of the planters. Label all of the planters as to identify the soil in each planter with a black Sharpie® Extreme black marker. Water the grass two times a day, once at 8 a.m. and again at 12:30 p.m. with 1.5 ml of distilled water using a disposable pipet. After the 8 a.m. watering, place the planters into the greenhouse.
The zinc will form a new compound with the sulfate, and the copper will stay as a metal. Balanced Chemical
4. Remove the supernatant and transfer the pellet to a 1.5 mL microcentrifuge tube. 5. Suspend the pellet in eosin to make the spheroids more visible. 6.
Chocolate Agar- Used for capsule demonstration. When subculture are to be done on this medium and incubated at 37oC in a candle jar with raised pCO2 mucoid colonies were obtained after 24 hours (Perfect JR and Cox GM, 2005). Colony morphology C. gattii and C. neoformans grow readily on SDA. Colonies
Its pH is greater than 7 and turns red litmus paper into blue. Acid- base neutralization is done by adding an acid to a base or a base to an acid until the substance has equal hydrogen and hydroxide ions. This is used to determine unknown concentration of a
The first step to check aquatic nitrate was to rinse the pipette, fill it up with 5mL of water from the aquatic section and put it in a test tube, the next thing they had to
Preparing staining buffer ……………………………………………………………………9 Staining Reagents and Supplies………………………………………………………….... 9 Staining Procedure………………………………………………………………………...... 10 Troubleshooting………………………………………………………………………….. ….11 Reference……………………………………………………………………………………… 12
