The calpain and calpastatin enzymes system plays a role in protein muscle renewal, as well as in the process of postmortem proteolysis of the meat in cattle. Tenderness of the meat depends on the magnitude of this process. The main enzyme is Calpain whereas Calpastatin is its inhibitor; numerous studies have identified diverse polymorphisms of the genes that code for both enzymes. Polymorphism CASTt1 and CASTuog are two mutations on the calpastatin gene, which have been identified as potential forecasters of meat tenderness. The objective of this study was to supplement previous studies, realized at the Veterinary Science Faculty, about genotyping for Calpain gene with the allelic and genotypic polymorphism frequency of CAST1 or CASTuog in