ipl-logo

Citrate Test Lab Report

1589 Words7 Pages

Results Gram Stain: The Gram Stain test was performed on our unknown bacteria F#1729 resulting in in a Gram-negative (-) result meaning that it contained a thin layer of peptidoglycan. This thin outer membrane is composed of lipopolysaccharides and cannot retain the crystal violet iodine used, instead it sloughs off when rinsed with the ethanol alcohol. This leaves the color unretained and the counterstain safranin is absorbed, staining the bacteria pink. Once the slide was ready, we were able to observe the bacterial morphology under the microscope. We identified the bacterial shape as bacillus under 100X magnification with oil immersion. Figure 1: (Pseudomonas Aeruginosa mixture, Under 100X oil immersion) Oxidase Test: To test for the …show more content…

The growth can be observed visually by the color change of the pH indicator. The bacteria is inoculated onto the agar slant and incubated for a period of 24 hours at 37 degrees Celsius. If the results are negative, there is no color change and the medium stays green as shown on Figure 5 (A). However, if there is a color change from green to blue as seen in Figure 5 (B), then the results are positive. After performing the test and incubated the bacteria for 24 hours, we observed that our unknown bacteria showed positive results meaning that it is able to use citrate as its sole source of carbon for …show more content…

The next test that needed to be performed according to the dichotomous key was the oxidase test. If the results of the Oxidase test showed positive results, this would mean that our unknown bacteria could be aerobic and can use oxygen as a terminal electron acceptor in the respiration process because it would contain the cytochrome enzyme. If the results showed negative, our bacteria would not contain the cytochrome enzyme meaning that the bacteria could still be a facultative anaerobe, anaerobic, or aerobic. After the test was performed, the result determined that it was positive suspecting that the bacteria could be P. aeruginosa but further test was needed to be done to be

Show More
Related

Bacillus Megaterium Lab Report

914 Words | 4 Pages

INTRODUCTION AND BACKGROUND Bacillus megaterium is a gram positive, endospore forming, rod shaped bacteria, motile, and it is not capable of butanediol fermentation. It is considered aerobic and one of the first bacteria's genome that has been fully coded. It is found in soil and considered a saprophyte. Bacillus megaterium is Latin for the big beast because it is an enormously large bacteria. It is about 100 times as large as E. coli and due to its massive size, (about 60 micrometers cubed), Bacillus megaterium

Read More

Alcaligenes Faecalis Experiment

636 Words | 3 Pages

We got negative for indole (no production of indole, pyruvic acid and ammonia), negative for Methyl Red (our bacteria does not perform mixed-acid fermentation when supplied glucose), negative for Voges-Proskauer (no fermentation of glucose in order to produce 2,3-Butanediol-Butanediol fermentation), but positive for Citrate utilization, which means our bacteria uses citrate as a sole carbon source and energy. Something interesting here is that according to the lab textbook organism that degrade citrate must also use ammonium salts, and in the process, they produce ammonia that causes the medium to become alkaline (under this condition the medium turns to deep Prussian blue, indicating the utilization of citrate). The genus Alcaligenes is well known for being alkali-producing

Read More

Lab Report: Citrobacter Aerogenes, Staphyloccus

513 Words | 3 Pages

The agent used was called tetramethyl-p-phenylendiamine. When reduced the agent was clear but when oxidized it turned deep purple to a blackish color. This agent (Cytochrome C Oxidase) was the electron donor. This test did not distinguish whether the organism is oxidative or if it fermented.

Read More

Exercise 14 Unknown Identification Lab Report

449 Words | 2 Pages

Since unknown organism B was gram positive, I had decided my first biochemical test would be the Catalase test per lab manual. The result of the catalase test after adding H2O2 was immediate air bubbles. The second biochemical test performed on organism B was starch agar per lab manual. Once the reagent was added I did not

Read More

Unknown Lab Report

1706 Words | 7 Pages

The student is to use studied tests to eliminate possible generas and to isolate the correct genus and identify it to the given unknown. This proves the diversity of microorganisms and their uniquely varying traits. The resulting data obtained in class will enhance students’ laboratory skills and knowledge regarding microbial laboratory. Introduction

Read More

Petri Dish Lab Report

1940 Words | 8 Pages

The experiment began by obtaining a sterile cotton swab and a petri dish and searching for a collection site in the Biology Department that would have bacteria. The unknown sample was collected at a recycling bin in Biology Building East. We swabbed the recycling bin using the sterile cotton swab and gently streaked the swab across the surface of the petri dish and immediately covered it with the lid. The petri dish was then placed in an incubator at 37C for two days. Two other petri dishes were obtained air and skin.

Read More

Lab Report Unknown Bacteria

489 Words | 2 Pages

The agar did not change color from pink to yellow, meaning that no mannitol fermentation occurred. After comparing the result of no mannitol fermentation to Bergey’s Manual, as well as the other test results, it was possible to conclude that the gram positive bacteria was Micrococcus

Read More

Microbiology Unknown Lab Report

1556 Words | 7 Pages

The Cysteine desulfurase test implies that the bacteria can hydrolyze cysteine to produce hydrogen sulfide. Lastly, the Citrate utilizing enzymes test came back positive which indicates that the bacteria has the ability to use citrate as a sole carbon energy source for growth and to produce alkaline

Read More

Micrococcus Luteus Lab Report

1340 Words | 6 Pages

Using aerobic respiration, water and CO2 are produced. Occasionally hydrogen peroxide is formed in the place of water. Common enzyme in bacteria, Catalase is detected by adding a small amount of hydrogen peroxide to the sample of the unknown bacteria. If it is present, there will be a reaction of bubbles. There is positive reaction to this test.

Read More

Eukaryotes Lab Report

1884 Words | 8 Pages

Scientists begin to study microorganisms in order to avoid disease events such as the typhoid, small pox and cholera outbreaks. Research of microbiology evolved over time with the development of technology. Biochemical and molecular techniques have become vital in the identification of bacteria. The techniques have become—quicker, easier, affordable and accurate. Labs are now able to sequence bacterial DNA which has provided more information about microorganisms which

Read More

Pseudomonas Aeruginosa Research Paper

594 Words | 3 Pages

The bacteria, Pseudomonas Aeruginosa, is gram- negative. This means it is does not have a violet stain. It is also considered a bacillus bacteria. Pseudomonas aeruginosa can be strictly found in aerosols, this means it is compressed in pressure and released into a fine spray. Research has been made and found that this bacterium harms a lot of plants, animals, but mainly humans in such case.

Read More

Deep Agar Lab Report

218 Words | 1 Pages

From to the result we gain from these experiment, when we transfer microbes (E. coli) from slant to the deep agar by using stab wire, the shape of microbe form is filiform. Besides the shape filiform we get in the test tube, there is also some sort of crack on the agar that shows respiration occur in the agar. Usually, E. coli are remain viable but the oxygen availability is at the minimum rate that make the rate of growth of bacteria at slow pace. The result is taken after the test tube that contain the stab agar was incubated at 37° C for 24 hours. Microorganism shows great deal when it comes to oxygen requirement.

Read More

2014 Ap Bio Lab Report

1124 Words | 5 Pages

We had in our tables some materials needed for this experiment that were: 4 petri dishes, cotton swabs, sterile water, spreader, alcohol lamps and marker for labeling the plates. Materials were used to examine the appearance, structure and the growth of bacteria in four different environments based on the instructions. Methods After the separation of students into the groups we have waited for approximately 2 days to let bacteria grow and than noticed the structure and appearance of bacteria with microscope. In this case, we used to collect all information and results for each plate of bacteria in its environment from other groups also. Results A) Environmental

Read More

Does Low Ph Level Affect The Growth Of Cyanobacteria And Algal

2549 Words | 11 Pages

The experiment is not valid as it was not reliable, precise, accurate, nor was it a fair test. To improve the experiment and the results, the experiment should have been repeated at least 3 times to ensure reliability and test for precision. The method should have speculated a way of measuring the changes in the growth of the Cyanobacteria and algal species, this would have ensured am accurate set of results. The experiment did not correctly take changes in pH levels into proper consideration, as the changes should have been recorded rather than regulated back to the specific original pH levels. If these changes to the method were taken into account, then more accurate scientific results would be produced.

Read More

Lab Report On Fermentation

1432 Words | 6 Pages

Then, tests are performed to determine if the products of aerobic and anaerobic respiration are present in the flasks. The citric acid cycle consists of a series of chemical reactions used by all aerobic organisms to release stored energy through the oxidation of acetyl-CoA derived from carbohydrates, fats, and proteins into carbon dioxide and chemical energy in the form of ATP (Biology). The tests detect the presence of carbon dioxide and ethanol. Carbon dioxide should be present irrespective of the type of respiration taking place, but ethanol is present only if fermentation has occurred. Another factor that can indicate whether fermentation occurred or cellular respiration occurred is the amount of glucose utilized during incubation.

Read More

More about Citrate Test Lab Report

Open Document