Comparision Of Different Cell Types And Video Images Of Live Blood Cells

Introduction: The purpose of this experiment was calibrate the CX31 light microscopes for quantitative microscopy and make measurements of different cells and organelles. An ocular micrometer is a ruler inside the eyepiece of the microscope and it allows one to measure the specimen (mm). The purpose of the second experiment was to be able to distinguish different cell types within human blood using prepared slides and video images of live blood cells. Another aspect of this lab was to observe a set of 50 leukocytes and compare the results with the percentages of different types of leukocytes found in the human body.

Materials/Methods: A CX31 light microscope was used to observe a collection of animal, plant, and blood cells. Both groups were also given the chance to make their own slide to look at with the Vitachrome Amoeba and Paramecium. In the second lab, students stained a blood smear slide with a Wright Stain and Buffer

In between the cluster of red blood cells, random purple cells or different shapes and sizes were found – lymphocytes, neutrophils, platelets, etc. The neutrophils were bigger purple circles that had four compartments of darker purple circles within them. The eosinophils were purple circles that had two compartments of two darker circles within them. The blood platelets were extremely small purple dots. The monocytes looked like a purple kidney bean. The lymphocytes were darker, opaque purple circles. The basophils are purple circles that have two compartments of two purple circles with small scattered purple circles around the cell. After identifying the different cell types within human blood, we calibrated the ocular micrometer in order take measurements of the different cell types. The average diameter of the different types of cells did not contain any outliers or numbers that skewed the