Diabetes Mellium Case Study

ABSTRACT

The objective of this long term study was to evaluate anti- diabetic activity of Artemisia absinthium( methanolic leaf extract of Artemisia absinthium - MLEAA) in Streptozotocin induced experimental diabetes mellitus in normal adult male Wistar rats via comparison of changes in body weight , levels of plasma glucose and insulin , among the empirical groups. About 32 experimental rats male were divided into four groups, eight in each group; Normal (N), Normal treated (NT) with MLEAA, Diabetic (D) and Diabetic treated (DT) with MLEAA. Experimental diabetes mellitus was induced by a single dose intraperitoneal injection of Streptozotocin (55 mg/kg body weight) in respective groups meant for Diabetic (D) and Diabetic Treated (DT).

Herb to product ratio was 8:1, the needed extract was suspended in 5% Tween-80 in distilled water prior to utilize.The extraction process followed by the company is; leaves of Artemisia absinthium were air dried under the shade for a week. 500g of dried leaves were powdered, sieved with mesh and extracted with 1.5L of methanol (80%) using soxhlet apparatus at 70°C for 5 hours. The extract was filtered and the filtrate was evaporated to dryness under reduced pressure at 60°C (20% w/w dry weight basis substratum - yield) and stored at 4 °C until used for oral

STZ is a poisonous glucose twin that piles up in pancreatic beta cells via glucose conveyor GLUT2. Once STZ enters into the cell; it suppress the glucose metabolism and insulin release from beta cells and impairs the pancreas. This leads to changes in body weight, huge elevation in blood and urine sugar levels and gradual fall in the insulin levels of the test animals.Body weight decreases in of diabetic rats due to defect in glucose metabolism and increase in muscle wasting. Though DT rats showed a gradual increase in body weight at the terminus of experimental period, the body weight of DT group was significantly lower than N group but significantly higher than D group. Thus, Artemisia absinthium administration for 60 days interrupted the weight loss in DT group compared to D group, and this may be due to improvement in insulin secretion and insulin action.The percent decrease in plasma glucose level of DT group in 60 days is because of Artemisia absinthium treatment respectively. Thus, the present study indicates that chronic treatment of diabetic rats for 2 months with Artemisia absinthium decreased the plasma glucose level to the near normal values. The plasma glucose level of NT and DT groups during the experimental period clearly indicate that Artemisia absinthium does not exhibit hypoglycemic activity; instead, it shows anti-hyperglycemic