Virgin Olive Oil Essay

Synergistic Analgesic, Anti-Pyretic and Anti-Inflammatory Effects of Extra Virgin Olive Oil and Ibuprofen in Different Experimental Models in Albino Mice.
Walla’a A. Osman1, Dina A. Labib1, Mona O. Abdelhalim1, Elsayed M. Elrokh1
1Department of Medical Pharmacology, Faculty of Medicine, Cairo University
Faculty of Medicine, Kasr Al Ainy st., post code:11562, tel/fax:(202):23682030, Cairo, Egypt,
Corresponding author:
*Dina Ahmed Aly Labib
Lecturer of Medical Pharmacology, Faculty of Medicine, Cairo University e-mail: [e-mail] Tel.: [phone]
Walla’a Abdelfattah Osman
Assistant lecturer of Medical pharmacology, Faculty of Medicine, Cairo University e-mail:[e-mail] Tel.: [phone]
Mona Osman

This study was carried out to assess the analgesic, anti-pyretic and anti-inflammatory activities of extra virgin olive oil (EVOO) at a dose of 8ml/kg body weight and to compare it with Ibuprofen as an individual drug therapy and in combination with two different doses of IBU (therapeutic dose 100mg/kg and low dose 40mg/kg), on different animal models in albino mice. A total of 132 adult healthy male Swiss albino mice were used in this study. The analgesic effect was assessed using acetic acid-induced writhing test. The antipyretic effect was evaluated by brewer's yeast-induced pyrexia. While, the anti-inflammatory activity was assessed by two different models; the carrageenan-induced paw edema and the carrageenan-induced peritonitis in which the levels of total leukocyte count (TLC), neutrophil count, prostaglandin E2 (PGE2) and interferon gamma (INF-γ) were measured in the peritoneal exudates. The results revealed significant protection in all the treated groups; however, the combination of EVOO with IBU at its therapeutic dose showed superiority over the two compounds when used separately. Conclusion: Using extra virgin olive oil with the therapeutic dose of ibuprofen showed synergistic effect in controlling the cardinal signs of acute inflammation rather than using the NSAIDs

Therefore, this work was designed to evaluate the analgesic, antipyretic as well as the anti-inflammatory effects of EVOO and compare it with ibuprofen individually and in combination with two different doses of IBU (therapeutic dose 100mg/kg and low dose 40mg/kg), on different animal models in albino mice. MATERIAL AND METHODS Animals: A total of 132 adult healthy male Swiss albino mice, weighting 25-30 g were bred in the animal house of Kasr Alainy hospital. The mice were left for 2 weeks for acclimatization before use. Animals were maintained at a constant temperature (24+1C) with 55% humidity, on a 12 hrs light/dark cycle. The night before the experiments, food was withdrawn from the cages but water was given ad libitum. The study protocol was in accordance with the guidelines for animal research and it was approved by the Institutional Animal Care and Use Committee (CU-IACUC), the approval number is (CU III S 4