Introduction Dried blood spot cards commonly abbreviated as DBS were first shown to public in 1963. It was a simpler way to find out whether the patients were suffering from metabolic diseases. It was especially helpful among large populations. Some of its advantages were immediately noticed. The advantages were clear against the traditional sampling techniques. One of the most talked about reasons was less invasive idea. However, there was also problem in terms of handling and storing the sample that was also solved. The method started being applied to numerous different procedures such as therapeutic drug monitoring or the pharmacokinetics studies. The main assumption regarding the DBS is that if the punches were measured correctly, it would …show more content…
These substrates include: glucose, palmitate, amino acids and propionate. The aim was to make sure that any type of blood spot card would be able to show the chemical structure of the chemicals on them. Therefore, four different sampling cards were chosen: FTA DMKA-A, FTA DMPK-B, VWR 237 and Protein Saver 903. These cards supposedly have different structures, so it would be possible to see which one was more successful with each of the chemicals named above. In order to be able to use these sampling cards, the instruments such as Harris Uni-core 3mm cutters were necessary. They also came with the cutting mats. The next major point was to prepare the actual samples of the chemicals listed above. In order to do this, the dried blood spots on different cards were punched and dispensed into the 108-well plates. 40 mL standard for the acetonitrile was then added to the mixture. Later, the uppermost layer was heated to ensure the nitrogen steam would evaporate and leave its traces on the paper. The next major step in the methodology was using the chromatography and mass spectrometry. Both of them were used in the settings given by the