Aim:
Therefore the aim of this experiment is to investigate the effect of increasing temperature on the activity of the enzyme catalyst.
Hypthesis:
The froth volume increases as temperature goes up until an optimum point of around about 40C and after that point the enzyme is denatured and the graph starts to decline. Experimental Procedures
Variables
The independent variable that is changed and controlled specifically to test the effect of the independent variable on the dependent variable is the temperature of the hydrogen peroxide and the liver, which affects the froth volume, which is the dependent variable.
Constant variables in this experiment was the size of the liver, the amount of hydrogen peroxide and the time the volume was measured. The size of the liver could increase the
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The volume of the froth kept rising until it was at its peak at 30 degrees and then the volume started decreasing. Hence the optimum temperature according to the results was 30 degrees with a volume of 62 ml.
The graph also has an optimum temperature of 30 degrees however the froth volume was 47.0.
Both figure 2 and 3 display a quadratic and have an optimum point. The graphs have the same nature as one another. The gradient of both graphs are positive then negative. However the values on both graph differ.
The volume of the froth at 10 degrees for the single data was 30 ml, while the volume for the average date at 10 degrees was 35.2 ml. The volume for froth at 20 degrees for the single data was 42 ml, while the volume for the average data was 40.5 ml. At 30 degrees the volume of the single data was 62 ml and the average data was 47.0 ml. At 40 degrees the single data had a froth volume of 35ml and the average data had a volume of 45.0ml. Finally at 50 degrees the volume of the froth for the single data was 32 ml and the average data was