LABORATORY REPORT Activity: Enzyme Activity Name: Natalie Banc Instructor: Elizabeth Kraske Date: 09.26.2016 Predictions 1. Sucrase will have the greatest activity at pH 6 2. Sucrase will have the greatest activity at 50 °C (122 °F) 3.
LABORATORY REPORT Activity: Enzyme Activity Name: Natalie Banc Instructor: Elizabeth Kraske Date: 09.22.2016 Predictions 1. Sucrase will have the greatest activity at pH 6 2. Sucrase will have the greatest activity at 50 °C (122 °F) 3. Sucrase activity increases with increasing sucrose concentration Materials and Methods Effect of pH on Enzyme Activity 1. Dependent Variable amount of product (glucose and fructose) produced 2.
Introduction: Enzymes are needed for survival in any living system and they control cellular reactions. Enzymes speed up chemical reactions by lowering the energy needed for molecules to begin reacting with each other. They do this by forming an enzyme-substrate complex that reduces energy that is required for a specific reaction to occur. Enzymes determine their functions by their shape and structure. Enzymes are made of amino acids, it 's made of anywhere from a hundred to a million amino acids, each they are bonded to other chemical bonds.
How Does the Type of Enzyme Affect How Much Apple Juice is Extracted From Applesauce Question: How does the type of enzyme affect how much apple juice is extracted from applesauce? Hypothesis: If seven drops of pectinase, cellulase, and a mixture of both are stirred ten times into 100 mL of applesauce and left for 5 minutes, then pectinase will catalyze the apple juice the fastest. IV: Type of enzyme (pectinase, cellulase, mixture of both) DV: Amount of applesauce extracted as measured in mL after 5 minutes Materials Needed: 1.400 Grams of applesauce 2.Pectinase
Enzymes are proteins that significantly speed up the rate of chemical reactions that take place within cells. Some enzymes help to break large molecules into smaller pieces that are more easily absorbed by the body. Other enzymes help bind two molecules together to produce a new molecule. Enzymes are selective catalysts, meaning that each enzyme only speeds up a specific reaction. The molecules that an enzyme works with are called substrates.
Introduction When a chemical reaction occurs anywhere in the universe, it needs energy. The human body is no exception. For some reactions, the energy required to start the reaction is Enzymes are special proteins designed to assist in the breaking down of macromolecules. They do so by holding the macromolecule in place at the active site, therefore lowering the amount of energy it takes to start the chemical reaction. There are different enzymes for each macromolecule; Pectinase and Cellulase are both examples of enzymes, and were the enzymes tested in this lab.
Introduction: Enzymes are biological catalysts that increase the rate of a reaction without being chemically changed. Enzymes are globular proteins that contain an active site. A specific substrate binds to the active site of the enzyme chemically and structurally (4). Enzymes also increase the rate of a reaction by decreasing the activation energy for that reaction which is the minimum energy required for the reaction to take place (3). Multiple factors affect the activity of an enzyme (1).
Enzymes are biological catalysts made of proteins that accelerate chemical reactions by lowering their activation energy therefore increasing the activity rate of the enzyme and more substrates turned into products. The ‘Catalase’ enzyme that was used during this experiment was obtained from peroxisome found in celery which are organelles found in bacteria, plant, and animal cells. It is involved in the breaking down of certain substances and the diminish of reactive oxygen species and that includes hydrogen peroxide (H2O2) which can be a byproduct of the metabolism of oxygen. Hydrogen peroxide is toxic to the cell and so the catalase enzyme is utilised to break down H2O2 to form oxygen molecules and water free of free radicals.
An enzyme is a biomolecule that acts as a catalyst in biochemical reactions (1). Enzymes are commonly used in many products and medications. Enzymes function by flexibly binding to active sites in substrates (reactants). This binding is weak non-covalent interactions.
They can only quicken reactions that will eventually occur, but this enables the cell to have a productive metabolism, routing chemicals through metabolic pathways. Enzymes are very specific for the reactions they catalyze; they make sure the chemical processes go in the cell at any given time. Peroxidase was the enzyme being testing in this experiment. A peroxidase is an enzyme that acts as catalysts, which occurs in biological systems. Peroxidase is found in plants, which they play a role in helping to minimize damage caused by stress factors or insect pests.
Enzymes: Enzymes are the biological catalysts which are globular proteins in nature. They catalyze or accelerate the chemical reactions. They speed up the chemical reactions by providing an alternative reaction pathway of lower activation energy. The molecules on which enzymes acts are called substrates and these are converted into different molecules called products. All the metabolic processes in the cell need enzymes to occur at a faster rate to sustain life.
In 1856, a scientist name claude Bernard has identified lipase [1]. Lipases are serine hydrolases containing G-X1-S-X2-G sequences as the catalytic part of the particle, where G = glycine, S = serine, X1 = histidine, X2 = glutaminic or aspartic acid. Such structure is characteristic also for serine proteases. The knowledge of their 3-dimensional structure plays a significant role in designing and structuring lipases
Enzymes are reliant on their structure to perform their tasks, so when the structure of the enzyme is changed it can no longer perform its task. When the enzyme can no longer perform its function due to a change in structure, the enzyme has been denatured(Meyertholen, Edward). To test whether processing foods effects enzyme activity, bromeliads and jello
SPECIFIC ACTIVITY MEASURING ENZYME KINETICS Enzymes are proteins that act as catalysts in reducing the amount of activation energy for a reaction to take place, activation energy that is needed for get reactions begins since several reactions. Do not take place at all. If they thermodyanamically possible. Enzymes catalyse specific reaction in a particular site known as the active site.
All substrates, if not specifically stated, are designated as A,B,C and D. All products are designated as P,Q,R. Different forms of enzymes are represented by E,F,G. E is the form of the enzyme that is free or at least most nearly free of any form of the substrates or products. Kineticists recognize two general mechanism for multisubstrate reactions: Sequential Mechanisms Non Sequential Mechnisms 1. Sequential Mechanisms
