ipl-logo

Biifidobacterium Dentium Bd1 Lab Report

1586 Words7 Pages

Objective - Functional Metabolic Map of Bifidobacterium Dentium Bd1, an Oral pathogenic bacteria Using High Quality Genomic Scale Metabolic Reconstruction.

Abstract

We have taken into consideration a class of bacteria that is one of the beneficial probiotic class bacteria. The reason for the choice of the bacteria is that it causes dental caries in children. It is reported to be one of the primary reasons for dental caries in children. This study was carried out in order to study its genetic answer to the pathogenicity. We present a metabolic model for the Bifidobacterium dentium Bd1 to answer some underlying questions. A mathematical model is created for the analysis of its metabolic network. We were able to find essential genes which take part in biomass production. As a result we have constructed this very first generic metabolic model for this organism that could simulate growth, by product formation …show more content…

All of these components are used in a method called flux balance analysis (FBA) to simulate microbial metabolism in a specified environmental condition. All of these components are used in a method called flux balance analysis (FBA) to simulate microbial metabolism in a specified environmental condition. FBA involves the use of linear optimization to define the limits on the metabolic capabilities of a model organism by assuming that the interior of the cell exists in a quasi-steady-state.

These mass balance constraints and reaction flux bounds form a set of underdetermined linear equations with many possible solutions. Maximum growth yield is simulated by maximizing the flux through the biomass reaction in the model, while the uptake of nutrients is fixed at a specific ratio. This is a meaningful optimization criterion because organisms have been observed to grow at the maximum predicted yield when nutrients are

Show More
Related
2033 Words | 9 Pages

Chem 51LB Report Ngoc Tran - Student ID # 72048507 The purpose of this lab is to examine the composition of three components of gas products of elimination reaction under acidic condition by conducting the dehydration of primary and secondary alcohol, and under basic condition by conducting the base-induced dehydrobromination of 1-bromobutane and 2-bromobutane. Then gas chromatography is used to analyze the composition of the product mixtures. Gas chromatography (mobile phase) is used to analyze the composition of three components of the gas products. A syringe needle with gas product is injected into the machine, and the component is eluted and the composition is related to the column or the peaks.

Read More
914 Words | 4 Pages

INTRODUCTION AND BACKGROUND Bacillus megaterium is a gram positive, endospore forming, rod shaped bacteria, motile, and it is not capable of butanediol fermentation. It is considered aerobic and one of the first bacteria's genome that has been fully coded. It is found in soil and considered a saprophyte. Bacillus megaterium is Latin for the big beast because it is an enormously large bacteria. It is about 100 times as large as E. coli and due to its massive size, (about 60 micrometers cubed), Bacillus megaterium

Read More
735 Words | 3 Pages

On pages 5-6, the diffusion coefficient of growth factor in water D is quoted from literature, whilst for growth factor concentration C*, duration of cells differentiation phase T, and the cell death rate Γwn were merely taken from personal communication with an independent laboratory. The rate of cell proliferation Γnw, cell differentiation Γud, solute uptake ΓR1 and Michaelis-Menten uptake K were selected to fit experimental observations received in the same manner. These data are proven [1, 2] to deviate between cell types and in response to culture environmental variations. Authors are advised to either publish detailed experimental data in the appendices, or consult reviewed articles, then assess the sensitivity of the model to different species and perfusion

Read More
1054 Words | 5 Pages

Identification of bacteria within Unknown Culture #21 In this experiment, an unknown culture of two different types of bacteria was assigned to each person, a number of tests were performed to isolate and identify these bacterial cells. Based on knowledge from the previous experiments completed in lab, a basic understanding of each type of bacteria was used to create a flow chart that would aid the process of identifying the unknown bacteria within the culture. A gram stain that is performed initially will narrow down the types of tests certain bacteria will and will not respond to. In addition to the gram stain, some of the tests that were used include, a catalase test, an Eosin methylene blue (EMB) agar test, a bile esculin test, and a 6.5% sodium chloride (NaCl) test.

Read More
1596 Words | 7 Pages

I expect to learn the biochemical differences in bacteria from this lab. Also, how to identify different species of bacteria. Material & Methods For the first day of the practical, an unknown specimen was provided

Read More
1149 Words | 5 Pages

Gastroenterology Research & Practice, 1-6. doi: 10.1155/2016/2687605 Nanwa, N., Sander, B., Krahn, M., Daneman, N., Lu, H., Austin, P., Govindarajan, A., Rosella, L., Cadarette, M., & Kwong, J. (2017). A population-based matched cohort study examining the mortality and costs of patients with community-onset clostridium difficile infection identified using emergency department visits and hospital admissions. Plos ONE, 12(3), 1-13. doi:10.1371/journal.pone.0172410 Ng, K., Ferreyra, J., Higginbottom, S., Lynch, J., Kashyap, P., Gopinath, S., Naidu, N., Choudhury, B., Weimer, B., Monack, D., & Sonnenburg, J. (2013). Microbiota-liberated host sugars facilitate post-antibiotic expansion of enteric pathogens.

Read More
407 Words | 2 Pages

This lab uses a lake simulation to study how the addition of nutrients and toxins can affect the lake, its inhabitants and the surrounding area. There is a strong focus on the addition of Phosphorous and added toxins because both are key elements in growth in lakes. The Virtual lake includes these five simulated species; green algae, cyanobacteria, bosmina, daphnia, and trout. There are two types of phytoplankton in the lake model, green algae and cyanobacteria. Green algae are a very diverse group that are photosynthetic, aquatic, plant like organisms that have a very simple reproductive structure.

Read More
1111 Words | 5 Pages

The goal of the experiment is to synthesize a bromohexane compound from 1-hexene and HBr(aq) under reflux conditions and use the silver nitrate and sodium iodide tests to determine if the product is a primary or secondary hydrocarbon. The heterogeneous reaction mixture contains 1-hexene, 48% HBr(aq), and tetrabutylammonium bromide and was heated to under reflux conditions. Heating under reflux means that the reaction mixture is heated at its boiling point so that the reaction can proceed at a faster rate. The attached reflux condenser allows volatile substances to return to the reaction flask so that no material is lost. Since alkenes are immiscible with concentrated HBr, tetrabutylammonium bromide is used as a phase-transfer catalyst.

Read More
865 Words | 4 Pages

How Pseudomonas “Sugar-coats” Itself to Cause Chronic Lung Infection Introduction- I had two infections, but in the article that I read, it did not discuss how to treat infections, but it discussed how one type of infection- a lung infection- can be caused- by pseudomonas “sugar-coating” themselves. Point 1- Pseudomonas rarely succeed at causing a chronic lung infection in humans, but there is one exception- cystic fibrosis patients. Point

Read More
1038 Words | 5 Pages

After my Bachelor of Science in Physics at Emory, I worked as a research assistant as I investigated the components of cellular function using Escherichia coli as a model organism in Dr. Minsu Kim’s research laboratory. Through manipulation at the molecular level, (i.e., altering genes, proteins, and metabolites to induce a synthetic biological system) we characterized certain functions of the cells by first understanding each individual component. To understand the relationship between each component and a certain function of the cell, we used quantitative experiments to bridge the biological processes at the molecular and cellular level using Biophysics techniques and mathematical modeling. My investigation included growing cells in minimal media with different strains of NCM 3722 E. coli, and I conducted viability assays to determine the death rate of cells after they reached stationary phase. Using minimal media, which is carbon and nitrogen free, we can manipulate the amount of carbon or nitrogen source, hence, the cell density in each culture.

Read More
990 Words | 4 Pages

Enterococci can survive in monoculture, but cause only minor lesions. The outer membrane of Gram-negative bacteria contains endotoxin, which is present in all necrotic teeth with periapical lesions, and is able to trigger an inflammatory response even in the absence of viable bacteria. Furthermore, the levels of endotoxin in necrotic root canals are positively correlated to clinical symptoms such as spontaneous pain and tenderness to percussion.85 Virulent Gram-negative anaerobic rods rely on the presence of other bacteria in their environment to survive and establish their full pathogenic potential. Such collections of microorganisms in an extracellular polysaccharide matrix associated with a surface (the inner root canal wall) are called biofilms. There is convincing evidence that microorganisms organized in this manner are far less susceptible to antimicrobial agents than their planktonic counterparts, which have been used by tradition to check the antimicrobial efficacy of substances in

Read More
1353 Words | 6 Pages

It is thought that the most prevalent source of such microorganisms is from the mother, although the exact mechanism of transmission is not well understood. Once acquired, a source of carbohydrates such as sucrose or fructose feeds the bacteria, which reduce the pH in the mouth as they proliferate, leading to demineralization of the enamel and dentin. The disease typically manifests in areas that are not commonly associated with later onset caries, most notably the smooth surfaces of the maxillary incisors. As the condition progresses, it spreads to the canines and molars, propagating to the mandibular teeth, with the mandibular incisors typically the last and least affected, due to their proximity to the primary salivary glands and protection from the substrate provided by the nipple of the bottle or the spout of the sippy

Read More
844 Words | 4 Pages

On top of this, group those plants with similar nutrient and water needs in the same

Read More
1780 Words | 8 Pages

When there are conditions of self-growth they can face these factors more efficiently (Corey

Read More
1780 Words | 8 Pages

Introduction Dentin bonding or adhesion is the micro-mechanical union of restorative materials to dentin, particularly dental composites, via an intermediary adhesive resin layer. Kramer and McLean (1952) were among the first to use glycerophosphoric acid dimethacrylate (GPDM) to bond to dentin. They notice altered staining of the tissue surface during histologic examination of the area that is presently known as the hybrid layer. Over the last 40 years, major advances have been made in both adhesive monomer formulations and in pre-treatment of dentin to improve resin penetration into the tissue matrix. Definitions ADHESION Adhesion refers to the attraction between the atoms and molecules at the contacting surfaces of different materials.

Read More

More about Biifidobacterium Dentium Bd1 Lab Report

Related Topics

Open Document