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Biifidobacterium Dentium Bd1 Lab Report

1586 Words7 Pages

Objective - Functional Metabolic Map of Bifidobacterium Dentium Bd1, an Oral pathogenic bacteria Using High Quality Genomic Scale Metabolic Reconstruction.

Abstract

We have taken into consideration a class of bacteria that is one of the beneficial probiotic class bacteria. The reason for the choice of the bacteria is that it causes dental caries in children. It is reported to be one of the primary reasons for dental caries in children. This study was carried out in order to study its genetic answer to the pathogenicity. We present a metabolic model for the Bifidobacterium dentium Bd1 to answer some underlying questions. A mathematical model is created for the analysis of its metabolic network. We were able to find essential genes which take part in biomass production. As a result we have constructed this very first generic metabolic model for this organism that could simulate growth, by product formation …show more content…

All of these components are used in a method called flux balance analysis (FBA) to simulate microbial metabolism in a specified environmental condition. All of these components are used in a method called flux balance analysis (FBA) to simulate microbial metabolism in a specified environmental condition. FBA involves the use of linear optimization to define the limits on the metabolic capabilities of a model organism by assuming that the interior of the cell exists in a quasi-steady-state.

These mass balance constraints and reaction flux bounds form a set of underdetermined linear equations with many possible solutions. Maximum growth yield is simulated by maximizing the flux through the biomass reaction in the model, while the uptake of nutrients is fixed at a specific ratio. This is a meaningful optimization criterion because organisms have been observed to grow at the maximum predicted yield when nutrients are

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