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Gram Negative Unk Lab Report

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Discussion and Conclusion The Gram positive Unk. A-DB was identified to be B. Cereus. This inference was reached though quite a bit of study. Firstly the Gram positive was found to be Bacilli shaped this eliminated the gram negative bacteia and three of the nine possible bacteria, leaveing B. cereus, B. megaterium, B. subtilis, C. diphtheriae, M. phlei and M. smegmatis. A timperature test was proformed so as to eliminate the two bacteria whose optimale temperature is 30°(alt+0176) C or exclude the bacteria that have 37° C as their optimale temperature. The temperature test resulted in the elimantation of B. megaterium and B. subtilis and leaving B. cereus, C. diphtheriae, M. phlei, and M. smegmatis as possible bacteria. The SIM’s test was …show more content…

A-WB was identified to be A. faecalis. This inference was reached though quite a bit of study. Firstly the Gram Negative was found to be Bacilli shaped this eliminated the gram Positive bacteia leaving A. faecalis, E. aerogenes, E. coil, K. pneumoniae, P. mirabilis, P. vulgaris, P. aeruginosa, S. typhi, S. marcescens, and S. flexneri. A SIM’s test was proformed on the unknown because it was one of the test that gave the most information while eliminating other bacteria. The SIM’s test results were positive for motility. the fact that the bacteria was motile eliminated K. pneumonia and S. flexneri, leaving A. faecalis, E. aerogenes, E. coil, P. mirabilis, P. vulgaris, P. aeruginosa, S. typhi, and S. marcescens as possible resultes. The SIM’s test also yielded negative result for sulfure reduction thus eliminatine P. mirabilis, P. vulgaris and S. typhi, leaving A. faecalis, E. aerogenes, E. coil, P. aeruginosa, and S. marcescens. The next test done was a glucose test because it would cut the number of possible bacteria almost in half. The results of the glucose test was a negative eliminating E. aerogenes, E. coil, and S. marcescens leaving only two bacteria A. faecalis and P. aeruginosa. P. aeruginosa is positive for both urease and gelatinase while A. faecalis is negative for both enzymes. Both tests were done on Unk. A-WB and resulted in negative results proving that Unk. A-WB was A. …show more content…

A. faecalis is normally considered a nonphathogenic bacteria; although some opportunistic infections occur. When opportunistic infections are causes by A. faecalis they are generally seen as urinary teact infection. A. faecalis is important to the medical field due to it’s contrabution to the prodution of nonstandard amino acids. Bacillus cereus is in the Bacillaceae family it is commonly fould in the food or soil. Some types or strains of B. cereus can be very dangerous to people and cause cases of food poisonings such as fried rice syndrome. While other types of B. cereus can be benificial to animals in the form of probiotics which is what make Bacillus cereus an important bacterium to the medical

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OBJECTIVES To solicit for aid to help study the biological importance of bacillus megaterium and to determine whether bacillus megaterium is susceptible or receptive to antibiotics by investigating the characteristics of the genus species and also. SUMMARY The purpose of this experiment is to be able to identify Bacillus megaterium using 16S PCR & Sequencing and also determine whether bacillus megaterium is susceptible or receptive to antibiotics using gram stain. Being able to determine whether bacillus megaterium is susceptible or receptive to antibiotics is critical to medical advancement and biotechnological enhancements.

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According to the series of test that my group ran for our unknown specimen, we had a match with the bacteria known as Alcaligenes Faecalis. This bacterium belongs to one of the major group of gram-negative bacteria (Phylum Proteobacteria). Alcaligenes Faecalis (Genus, species) is a rod shaped (bacillus), 0.5-1.2 x 1.0-3.0 µm, round with scalloped margin (colony configuration growth), motile (with one to nine peritrichous flagella), gram-negative, non-fermentative bacteria, obligate aerobic, having oxygen as the principal terminal electron acceptor in the electron transport chain (ETC). We consider we have a match with the species Alcaligenes Faecalis because of the following reasons: Fermentation tests performed (Durham sugars) were negative, which indicate that our bacteria use a different metabolic means for growth (non-fermentative gram-negative bacteria).

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The six bacteria used in this lab were, Citrobacter freundii, Bacillus subtillis, Enterobacter aerogenes, Staphylococcus epidermis, Enterococcus durans, and Escherichia coli. Citrobacter freundii is a Gram-negative rod shape bacteria. The MSA plate will grow Citrobacter freundii, Bacillus subtillis, Enterobacter aerogenes and will have a yellow color change while Staphylococcus epidermis will not grow nor have a color change to yellow. The MacConkey agar will have growth with Escherichia coli, Enterococcus durans, but not Staphylococcus epidermis and Bacillus subtilis since it is a Gram-positive.

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The second ½ of the organism was used for gram staining. The gram stain method was performed on the unknown organism per lab manual page 42 and two gram stain reactions were identified. Organism B was gram positive cocci in grape like clusters. Because organism B was positive I could eliminate Escherichia coli, Enterobacter aerogenes and Proteus vulgaris because these bacteria would be rod shapes. Organism A was gram negative pink rod shaped and because of gram positive morphology I could eliminate Staphylococcus aureus, Streptococcus lactis and Bacillus subtilis.

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The unknown bacteria was then tested on multiple selective and differential media. Growth was present on the MacConkey Agar and the colonies were the same color as the plate, which told me my bacteria was gram negative and did not ferment lactose. There was no growth on the Mannitol Salt Agar, and this told me the unknown was not salt tolerant and did not

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The bacteria were not transformed and no growth was present in either

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The percentage prevalence of the bacterial isolates was as follows- Staphylococcus aureus 18/63 (29%), Pseudomonas aeruginosa 14/63 (22%), Coagulase negative staphylococci (CoNS) 11/63 (17%), Klebsiella pneumoniae 8/63 (13%), Proteus vulgaris 7/63 (11%) and

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The Another medium used was MAC, it is used to isolate and differentiate gram-negative organisms and it is a pink, dusty rose color. Lastly, the Citrate Slant is a green color and it was used as a differential test to examine enzymes. The media were inoculated at 37°C for 48 hours, then it was observed to determine the

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