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Effect of enzyme concentration on enzyme activity
Effect of enzyme concentration on enzyme activity
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More handpicked essays just for you.
Effect of enzyme concentration on enzyme activity
Effect of enzyme concentration on enzyme activity
Don’t take our word for it - see why 10 million students trust us with their essay needs.
The effect of pH on the speed of enzyme interaction with substrate chemicals Hypothesis: About pH: If the pH level is less than 5, then the speed of the enzyme reaction will be slower. About temperature: If the temperature stays the same, then the speed of the enzyme reaction will not be completely affected. Background information: The function of enzymes is to speed up the biochemical reaction by lowering the activation energy, they do this by colliding with the substrate.
Cofactor- Molecules that aren’t proteins nor organic, but help make the reaction go faster when they connect to the active site. 9. competitive inhibitor- prohibits the reaction from taking place by going into the enzyme’s active site so the substrate can’t. 10.
It was hypothesized that the optimal pH for the enzyme was pH 7 while the 1.0 ml peroxidase would have the best reaction rate. At the end of the experiment the results prove the hypothesis to be incorrect. INTRODUCTION Enzymes are proteins that allow a reaction to speed up. These proteins are made up of monomers known as amino acids.
Enzymes speed up chemical reactions enabling more products to be formed within a shorter span of time. Enzymes are fragile and easily disrupted by heat or other mild treatment. Studying the effect of temperature and substrate concentration on enzyme concentration allows better understanding of optimum conditions which enzymes can function. An example of an enzyme catalyzed reaction is enzymatic hydrolysis of an artificial substrate, o-Nitrophenylgalactoside (ONPG) used in place of lactose. Upon hydrolysis by B-galactosidase, a yellow colored compound o-Nitrophenol (ONP) is formed.
Title: Enzymes Abstract: Enzymes can catalyze chemical reactions by speeding up the chemicals activation energy. Temperature and pH are just two of the factors that affects enzymes and their involvement with chemicals and the way they function. Throughout this experiment, we conducted a study on peroxidase, which is an enzyme. The following information consist of the recordings of when it was exposed to four different pH levels to come up with an optimum pH and IRV at the end. Introduction: Enzymes are proteins that are used in reactions in living organisms.
Introduction: Enzymes are needed for survival in any living system and they control cellular reactions. Enzymes speed up chemical reactions by lowering the energy needed for molecules to begin reacting with each other. They do this by forming an enzyme-substrate complex that reduces energy that is required for a specific reaction to occur. Enzymes determine their functions by their shape and structure. Enzymes are made of amino acids, it 's made of anywhere from a hundred to a million amino acids, each they are bonded to other chemical bonds.
Enzymes are proteins that significantly speed up the rate of chemical reactions that take place within cells. Some enzymes help to break large molecules into smaller pieces that are more easily absorbed by the body. Other enzymes help bind two molecules together to produce a new molecule. Enzymes are selective catalysts, meaning that each enzyme only speeds up a specific reaction. The molecules that an enzyme works with are called substrates.
In this multi-step experiment, there were multiple hypotheses which were stated. The first hypothesis was that if an enzyme catalase is added to Hydrogen Peroxide, then the temperature of the H2O2 will increase. The other two pertained to an enzyme being affected. If boiled catalase is mixed with Hydrogen Peroxide, then there will be a larger temperature jump than if only pure catalase was added. The third hypothesis was if catalase with acid is added to H2O2, then the temperature will have a smaller jump than if only pure catalase was added.
Introduction: Enzymes are biological catalysts that increase the rate of a reaction without being chemically changed. Enzymes are globular proteins that contain an active site. A specific substrate binds to the active site of the enzyme chemically and structurally (4). Enzymes also increase the rate of a reaction by decreasing the activation energy for that reaction which is the minimum energy required for the reaction to take place (3). Multiple factors affect the activity of an enzyme (1).
Enzymes display importance of structure, as when certain conditions affect the structure of the active site, the enzyme could denature, disrupting the whole reaction. Key processes such as DNA replication, respiration, and photosynthesis all of which involve enzymes, if the active site structure is not highly specific to the substrate, due to being denatured it affects the whole
An enzyme is a biomolecule that acts as a catalyst in biochemical reactions (1). Enzymes are commonly used in many products and medications. Enzymes function by flexibly binding to active sites in substrates (reactants). This binding is weak non-covalent interactions.
They can only quicken reactions that will eventually occur, but this enables the cell to have a productive metabolism, routing chemicals through metabolic pathways. Enzymes are very specific for the reactions they catalyze; they make sure the chemical processes go in the cell at any given time. Peroxidase was the enzyme being testing in this experiment. A peroxidase is an enzyme that acts as catalysts, which occurs in biological systems. Peroxidase is found in plants, which they play a role in helping to minimize damage caused by stress factors or insect pests.
Enzymes are biological catalysts that produce a constant supply of energy and help regulate the chemical reactions that occur in cells. Our daily lives depend on these types of proteins because without enzymes it can cause us severe illness or death and we would not be able to convert food into energy nor would our bodies be able to replace old tissues (Alberte, Pitzer, Calero, 2014). Protease and amylases are a type of enzyme that is produced by the body that helps break down starch and protein. With these enzymes, we are able to produce breaded products and we are able to brew alcohol, cheese, and detergents (Alberte, Pitzer, Calero, 2014). In respiration, enzymes contain an important role where aerobic respiration exhales the energy that
Digestion is a form of catabolism process of breaking down food physically and chemically large food molecules in to smaller components. Chemically digestion is carried out using enzymes and hormones with in different segments of the digestive tract. The presence of enzymes in the digestive tract helps breakdown polymeric biomolecules into individual monomers. This process is crucial for surviving because cells cannot use nutrients the way they were consumed without being metabolized. Nutrients need to be small enough to be absorbed by epithelium of the small intestine and transported by the help of carrier proteins.
An enzyme can be regarded as a catalyst for a biochemical reaction, or more simply, a biological catalyst. Its sole purpose is to increase the rate of a reaction, or speed up the reaction via provision of an alternative reactive pathway, which entails a lower activation energy. Enzymes participate in the reaction itself, enabling the occurrence of an alternate pathways of reaction, but they do not attain permanent changes to their structure or nature, and as a result remain unchanged preceding a reaction. It is then understood, that they can amplify the rate of a reaction, but are unable to effect upon the equilibratory position. Enzymes, unlike chemical catalysts are highly specific in terms of the reactions that they catalyse.