Synthesis Of Boiled Catalase

In this multi-step experiment, there were multiple hypotheses which were stated. The first hypothesis was that if an enzyme catalase is added to Hydrogen Peroxide, then the temperature of the H2O2 will increase. The other two pertained to an enzyme being affected. If boiled catalase is mixed with Hydrogen Peroxide, then there will be a larger temperature jump than if only pure catalase was added. The third hypothesis was if catalase with acid is added to H2O2, then the temperature will have a smaller jump than if only pure catalase was added. The above hypotheses were partially correct and partially incorrect. After adding catalase to Hydrogen Peroxide (HP), the temperature did increase rapidly, but then started to decrease in temperature. The second and third hypotheses were incorrect. In both cases, the temperature rose under a degree or did not rise at all, which contradicted the hypotheses.

The multiple experimental groups consisted of HP mixed with either normal enzyme catalase, catalase with acid, or boiled catalase. The pure catalase was largely different than the other two “affected” catalase, as it jumped from 23.5 °C to 33°C in one minute. After the the jump, it decreased at 0.5°C per minute, finally ending at 30°C. The other two experiments had mostly the same effect. The boiled catalase jumped 0.2 degrees in 30 seconds, then stayed for the rest of the time. The acid catalase remained at 23.5 degrees during the entire test, just as the pure H2O2