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Synthesis Of Boiled Catalase

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In this multi-step experiment, there were multiple hypotheses which were stated. The first hypothesis was that if an enzyme catalase is added to Hydrogen Peroxide, then the temperature of the H2O2 will increase. The other two pertained to an enzyme being affected. If boiled catalase is mixed with Hydrogen Peroxide, then there will be a larger temperature jump than if only pure catalase was added. The third hypothesis was if catalase with acid is added to H2O2, then the temperature will have a smaller jump than if only pure catalase was added. The above hypotheses were partially correct and partially incorrect. After adding catalase to Hydrogen Peroxide (HP), the temperature did increase rapidly, but then started to decrease in temperature. The second and third hypotheses were incorrect. In both cases, the temperature rose under a degree or did not rise at all, which contradicted the hypotheses. …show more content…

The multiple experimental groups consisted of HP mixed with either normal enzyme catalase, catalase with acid, or boiled catalase. The pure catalase was largely different than the other two “affected” catalase, as it jumped from 23.5 °C to 33°C in one minute. After the the jump, it decreased at 0.5°C per minute, finally ending at 30°C. The other two experiments had mostly the same effect. The boiled catalase jumped 0.2 degrees in 30 seconds, then stayed for the rest of the time. The acid catalase remained at 23.5 degrees during the entire test, just as the pure H2O2

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Nevertheless, the effects caused by the breakage of bonds will eventually lead to a decrease in the rate of reaction. As seen in the data, the reaction rate increased from 0.088 to 0.101 throughout the interval of -5℃ to 20℃ then decreased to 0.037 throughout the interval 20℃ to 56℃. This can be explained by the fact that 20℃ is the optimal temperature, therefore the active site of the enzyme is complementary to the substrate, causing the rate of reaction to be

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The addition of the extra catechol was supposed to have sped up the reaction with the enzyme and force the inhibitor out of the enzymes active site. If there was more time allowed to observe any possible color changes the results would have been more conclusive and our results more accurate. In other

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Although it was expected for water to be the optimal pH, it was also assumed that more drastic activity would happen with the other pH’s. For example, it was thought that it would still have some noticeable increase; however, when looking at the data and the graph, the numbers oscillate with no noticeable positive or negative trend. Tables 1 and 2 show that the absorbance rate in comparison to the absorbance rate in Table 3 are significantly smaller. Furthermore, after calculating the processed data for reaction rates and looking at the graph, pH 7 water had the highest rate. This experiment gives a good insight for future references about enzymes and the effect of environmental factors and its functions.

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7. Will the temperature effect the pH Scale? Hypothesis: The hypothesis of this experiment is that the rate of reaction will increases well as the hydrogen peroxide concentration. If the temperature, pH and enzyme concentration is kept constant then the rate of reaction will start to decrease as well as the hydrogen peroxide concentration. Aim: To investigate the effects of changing the concentration of the enzyme catalase that it has on the rate of breaking down the Hydrogen Peroxide solution.

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Most enzymes work best at body temperature of 37 (degrees Celsius). PH is another factor that affect the rate of enzymes activity. Enzymes function slowly in lower pH like in the pH of 1 or 2. Potato are high catalase when is being placed in a pH of 2 solution and hydrogen peroxide is added to it there will be some fizzing but not a good one. If the potato is placed in a pH of 13 and hydrogen peroxide is added there wouldn?t be any fizzing or little fizzing meaning that high pH either denature the catalase or substrate can not bind to the active site of an enzymes because its changes its shape.

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Enzymes are very valuable to the metabolic process. Enzymes are protein catalyst that lowers activation energy and speed up chemical reactions. Knowing what conditions affect enzymes will help further knowledge in what enzymes are and how they work. Catecholase was used in both experiments. The absorbance rate of each test tube was measured at 420 nm.

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These enzymes have a secondary and tertiary structure and this could be affected by increases and decreases in temperature beyond the optimum temperature of the enzyme to work in. Mostly enzymes are highly affected any changes in temperature beyond the enzymes optimum. There are too

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The enzymes are denatured because higher temperatures cause the enzyme

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If you conduct the catalyzed decomposition of hydrogen peroxide in a closed vessel, you will be able to determine the reaction rate as a function of the pressure increase in the vessel that is caused by the production of oxygen gas. If you vary the initial molar concentration of the H2O2 solution, the rate law for the reaction can also be determined. Finally, by conducting the reaction at different temperatures, the activation energy, Ea, can be calculated as heat is the energy source. Catalase is very efficient at decomposing hydrogen peroxide; one molecule of the enzyme can catalyse the conversion of over 6000,000 hydrogen peroxide molecules into water and oxygen every second. The enzyme occurs widely in tissues such as the liver and prevents accumulation of, and tissue damage by, hydrogen peroxide that is produced during metabolism.

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