Practical Activity Outline
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Recommended: Investigating the action of amylase
Predicting the Effect of pH on the Activity of Amylase
Arlecia Johnson
Enzyme to use:
Amylase is the easiest enzyme to find, it is in your saliva!
Source:
Saliva
Animal:
Human being
The substrate:
Starch, because amylases action starch and starch is also very easy to find in carbohydrate foods.
Method and type of treatment
A. Position a drop of solutions of iodine in test tubes (each with one drop) and place them in a row, in a test-tube rack.
B. Place labels on the test tubes that contain the pH to be used in the test.
C. Fill a syringe with amylase and drop 2 cm3 of it (from the syringe) into the test tube.
D. Take 1cm3 of a buffer solution (drop of a weak acid mixed with a portion of a strong
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