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Restriction Enzyme Digestion Lab Report

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Restriction Enzyme Digestion Four microtest tubes were acquired and LA and SD were written on all of the tubes. How to function a micropipette was introduced and 10uL of the buffer was added to all four tubes. In test tube one: 15uL of DNA 1, and 15uL of enzyme 1 were added. In test tube two: 15uL of DNA 1, and 15uL of enzyme 2 were added. In test tube three: 15uL of DNA 2, and 15uL of enzyme 1 were added. In test tube four: 15uL of DNA 2, and 15uL of enzyme 2 were added. The tubes were shaken and then put in a waterbath at 37o C to incubate for 50 minutes. After the incubation was finished, 5uL of solution to stop the reactions was added to all four tubes and they were stored in the refrigerator Casting and Loading a Gel for Electrophoresis
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