Tannase Agitation Rate

Agitation rpm level tannase production

Agitation rate was at a range of 50 to 250 rpm level was chosen to determine the optimal rate. It was found that an agitation rate of 100 rpm at pH 6.0 and 37°C maximum yield of tannase production of 3.12 U/ml (Fig.4) it was also noted that an increase in agitation speed above100 rpm resulted in a drastic fall in tannase enzyme production. The agitation speed below 100 rpm level resulted in an inadequate mixing of the broth towards of the broth. Towards the later stages of growth.

Effect of carbon and nitrogen sources

In the present study, addition of sugars as a potential carbon source did not have any positive effect on extracellular tannase enzyme production. It should be noted that degradation of tannic acid releases glucose and gallic acid, which can be efficiently utilized by the organism for its growth as a carbon source. Therefore, any disaccharide or polysaccharide that releases glucose on degradation will act as a better carbon source for tannase enzyme production (Bradoo et al., 1996). It has been reported that pure carbon sources, in general, inhibit tannase production, possibly indicating the presence of catabolite repression of protein biosynthesis (Aguilar et al., 2001). This was

cloacae are presented in Fig. 5b indicating that after 1 h of pre-incubation of the tannase enzyme, maintained a very high level activity of at pH 6 and (2.223U/ml), moderate loss of activity at pH 5.0 (1.761U/ml), but enzyme activity at pH 7 was at a considerable loss of activity tannase enzyme unit (1.618U/ml). The tannase enzyme was sufficiently active in the pH range of 6.0. Beyond this, the tannase enzyme lost most of its activity. Tannase from Paecilomyces variotii (Battestin and Macedo, 2007) have been reported to be culture conditions optimally at a pH range of 4.5–6.5 for 12 h with more than 80% relative activity. Tannase from Penicillium variabile was stable in the pH range of 3.0–8.0 for 24