Abstract: The purpose of this experiment was to identify given Unknown White Compound by conducting various test and learning how to use lab techniques. Tests that are used during this experiment were a flame test, ion test, pH test, and conductivity test. The results drawn from these tests confirmed the identity of the Unknown White Compound to be sodium acetate (NaC2H3O2) because there were no presence of ions and sodium has a strong persistent orange color. The compound then will be synthesized with the compounds Na2CO3 and HC2H3O2 to find percent yield.
Determining the Identity of an Unknown White Compound Maxwell Yurs Group Member: E.B. Floersch, Alexander Medina, and Masih Horri College Science and Engineering University of Minnesota, Minneapolis October 12, 2014 The experiments that were completed were used to determine the identity of an unknown white compound to allow for proper disposal. These experiments included flame tests, pH tests, and conductivity tests. After each was completed, it was found that the identity of the white compound was Sodium Nitrate.
According to the series of test that my group ran for our unknown specimen, we had a match with the bacteria known as Alcaligenes Faecalis. This bacterium belongs to one of the major group of gram-negative bacteria (Phylum Proteobacteria). Alcaligenes Faecalis (Genus, species) is a rod shaped (bacillus), 0.5-1.2 x 1.0-3.0 µm, round with scalloped margin (colony configuration growth), motile (with one to nine peritrichous flagella), gram-negative, non-fermentative bacteria, obligate aerobic, having oxygen as the principal terminal electron acceptor in the electron transport chain (ETC). We consider we have a match with the species Alcaligenes Faecalis because of the following reasons: Fermentation tests performed (Durham sugars) were negative, which indicate that our bacteria use a different metabolic means for growth (non-fermentative gram-negative bacteria).
On April 6, 2016 at approximately 11:45am, a local police station got a call about a hostage situation at a local pharmacy. When police and medical examiners got to each crime scene, they learned that all of the hostages were given drugs and had overdosed on them. Some of the pills, in powder form, were found near the victims. One of the victims was stable enough to tell the investigators that the power on the floor were the drugs they were forced to take. The medical examiner found out each hostage was given either unknown A or unknown B.
Exercise 14: Unknown Identification Lab Report The purpose of the study was to identify the unknown bacterium using various biochemical tests in addition to using scientific methods in determining the outcome of the hypothesis. Each biochemical test will help determine the bacteria based on specific characteristics of each organism. I was giving unknown number 232. The first procedure that needed to be done after obtaining unknown bacterial mixture was to isolate the two bacteria in a pure culture using the streak plate method described in Microbiology Laboratory Manual Eight Edition. The material used was trypticase soy agar (TSA) plate, nutrient plate, starch agar, hydrogen peroxide, iodine reagent and microscope.
While performing the slime lab, we were trying to find the effect of borax on glue and water. We mixed glue and water together and added small amounts of borax water to it. We wanted to find the difference in stretchiness with different amounts of borax. After adding the borax, we measured how far the slime could stretch. We hypothesized that the slime would stretch farther when more borax was added.
The objective of this experiment was to determine the identity of three unknowns existing in a 1:1:1 mixture. An extraction technique was therefore used to identify the unknown acid, base, and neutral as the compounds must first be separated. Extraction is a method of separation that is based on the difference in solubility of each compound in a mixture. There are a couple of necessary requirements for an extraction to be completed with experimental success. In this extraction there were two liquid solvents used, an original solvent (So), and an extraction solvent (Sx).
I don't accept my current grade, because it is wrong, and my grade should be higher than D in anyhow according to Dr.Scandale's grading policy. The following explanation is made on my behalf. Lab 1.1 grade = 0 "what should be the correct grade?", and if different, explain why
The experiment began by obtaining a sterile cotton swab and a petri dish and searching for a collection site in the Biology Department that would have bacteria. The unknown sample was collected at a recycling bin in Biology Building East. We swabbed the recycling bin using the sterile cotton swab and gently streaked the swab across the surface of the petri dish and immediately covered it with the lid. The petri dish was then placed in an incubator at 37C for two days. Two other petri dishes were obtained air and skin.
When Unknown Substance D was observed on the hot plate, it turned a brownish color and into a sticky liquid at two minutes just like the known covalent compounds, proving Unknown D had a low melting point. Ionic compounds cannot melt because they are made up of atoms with full opposite charges, so their intermolecular forces are stronger. While ionic compounds do not melt, covalent compounds do because their atoms only have slight charges, so their intermolecular forces are weaker. Since ionic compounds have stronger intermolecular forces, it takes more energy to pull the molecules apart. Opposite of ionic compounds, covalent compounds have weaker intermolecular forces, so it takes less heat to pull the molecules apart, which makes covalent
The first time the unknown bacteria was isolated, one of the colonies was observed to be bright yellow. This was important because it gave a clue as to which one was gram positive because looking for a yellow color was part of the gram positive dichotomous key. However, this conclusion that the yellow colony was positive and the white colony was negative was not certain until the conduction of the second gram stain. Also, even though Micrococcus was the only possible bacteria with colonies that have a distinctive pigment, all of the tests had to be performed to make sure that the other characteristics of this bacteria were consistent with the reported results that Micrococcus should have, according to the table. During the catalase test, the immediate bubbling of the bacteria was observed, meaning that it was catalase positive.
Introduction: Microbiology is the study of the microbial world, which is composed of microorganisms that are too tiny to be seen with a naked eye. This project demonstrates the significance of correctly identifying unknown organisms with methods and techniques acquired in the laboratory as they can make or break one's ability to accurately isolate and identify various species. Studying microbes enables oneself to have an insight on not just their structure or how they function, but how they have an impact on organisms and their environment around them as they can be found nearly anywhere. The importance of the process of identifying unknown bacteria vary from determining the causative agent of a disease, to testing if a specific food or fluid is safe to consume, and simply knowing what is present
The microbial world is extremely diverse. Every microbial species has a combination of morphological and metabolic characteristics. A series of biochemical test need to be carried out on an isolated culture of the unknown microbe. These tests will assess the metabolic properties of the unknown microorganism. Using the unique combination of characteristic determined by the series of tests, the unknown microbial species can be identified.
Streak plate The streak plate was successful due to the growth of colonies in the media. The unknown microbe was separated in small individual colonies. The colonies are light yellow in color, irregular shape, and shiny in texture. The successful separation of individual colonies is the foundation for many tests that will be used for the identification of the unknown bacteria.
8. After all of the characteristics were recorded we then looked at all of the slides again and began ruling out slides of the known cells that didn't appear to be similar. We narrowed it down between a protist and animal cell. We then compared the known and unknown cells under microscopes and looked further for more
