Enzyme inhibitor Essays

  • Cycloodiline Lab Report

    1066 Words  | 5 Pages

    2-aminopyrimidines as antitubercular & antidiabetic agents 1. Introduction: o The target name and type: The target in this paper is the mycobacterial di-hydro folate reductase, alpha-glocosidase and glycogen phosphorylase The type of the targets is enzymes. o Diseases that associated with the target: The diseases that associated with the target are diabetes and tuberculosis. o Biological activity of the compounds: Minimum inhibitory concentration (MIC): Susceptibility of the organisms which is isolated

  • Thymidylate Synthase

    1234 Words  | 5 Pages

    Case study 5 The design of an inhibitor for the enzyme thymidylate synthase is a representation for how de novo drug design and structure-based drug design can go hand-in-hand. In the human body, this enzyme uses the coenzyme, 5,10-methylentetrahydrofolate, to catalyze the addition of a methyl group to the substrate deoxyuridylate monophosphate (dUMP). The product of this reaction is deoxythymidylate monophosphate (dTMP). Because molecules that inhibit thymidylate synthase tend to display anti-tumor

  • Michaelis Menten Model Lab Report

    943 Words  | 4 Pages

    An enzyme is a biomolecule that acts as a catalyst in biochemical reactions (1). Enzymes are commonly used in many products and medications. Enzymes function by flexibly binding to active sites in substrates (reactants). This binding is weak non-covalent interactions. The Michaelis Menten model is used to show the relationship between velocity and substrate concentration, such as in figures four and five. Vmax is the maximum rate an enzymatic reaction can have. This is calculated along with Km, the

  • Factors Affect The Rate Of An Enzyme Catalyzed Interaction

    759 Words  | 4 Pages

    There are many factors which affects the rate of an enzyme catalyzed reaction. The rate of an enzyme controlled reaction is measured by 1.The amount of substrate change per unit time 2.The amount of product formed per unit time 3. The time taken for the completion of the reaction In investigating the effect of one factor : All the factors should be kept constant They must be maintained at suitable levels Only the initial rate should be measured. The factors affecting the rate of

  • Beta Galactosidase Lab Report

    494 Words  | 2 Pages

    Enzymes are proteins that significantly speed up the rate of chemical reactions that take place within cells. Some enzymes help to break large molecules into smaller pieces that are more easily absorbed by the body. Other enzymes help bind two molecules together to produce a new molecule. Enzymes are selective catalysts, meaning that each enzyme only speeds up a specific reaction. The molecules that an enzyme works with are called substrates. The substrates bind to a region on the enzyme called

  • Cuvette Enzyme Lab

    1834 Words  | 8 Pages

    substrate First, the blank was prepared according to table 2 without the enzyme addition. The enzyme was added later after the blank was measured by the spectrophotometer. Table 2: The amount of Sodium Phosphate Buffer pH 7.0, L-Dopa, and enzyme needed in each cuvette. Cuvette 1 Cuvette 2 Cuvette 3 Cuvette 4 Cuvette 5 Sodium Phosphate Buffer PH 7.0 (mL) 2.40 2.20 1.80 1.60 1.10 L- Dopa (mL) 0.20 0.40 0.80 1.00 1.50 Enzyme (mL) 0.40 0.40 0.40 0.40 0.40 For example, to prepare the cuvette 1, 2

  • Enzyme Catalyzed Reaction Lab Report

    1213 Words  | 5 Pages

    under ideal conditions and in the presence of an inhibitor. In a normal reaction, the reactant is converted to the product, whereas in an enzyme-catalyzed reaction, the substrate reversibly binds with the enzyme to form the enzyme-substrate complex, which is then converted to the product and the free enzyme. The rate of the reaction is the concentration of substrate consumed or the concentration of product formed, therefore, the rate of an enzyme-catalyzed reaction is the velocity at which the product

  • Amylase Lab Report

    755 Words  | 4 Pages

    Enzymes are biological catalysts, which are essential for carrying metabolic reactions in the human body including the breakdown of food for digestion, absorption and energy production. All biological reactions within human cells depend on enzymes (Wolfenden 1). It is essential for humans to have well-functioning enzymes to break down large molecules into smaller units. As a matter of fact, in the absence of normal functioning enzymes, the human body would cease to exist because chemical reactions

  • Enzyme Reaction Lab

    781 Words  | 4 Pages

    temperature, pH lever, and inhibitors of enzymes that increase the rate of a chemical reaction without making a change itself in living cells. In the experiments, peroxide was used as the enzyme. The hypotheses were: 1. Concentration of the extract would directly affect enzyme activity. 2. Rise in temperature would rise the rate of enzyme activity and absorbance until the temperature hits the optimal temperature, 3. pH level would affect enzyme activity, 4. and, the existence of inhibitor would decrease rates

  • Tyrosinase Lab Report

    1253 Words  | 6 Pages

    Introduction Tyrosinase is a copper-containing enzyme that catalyzes oxidation steps in melanin synthesis (Curto, et al.) Tyrosinase and its inhibitors have been studied for cosmetic and medical purposes. By inhibiting tyrosinase activity, skin can be whitened for cosmetic purposes. Inhibiting tyrosinase activity can also be a treatment for disorders involving elevated pigmentation of the skin such as age spots (Heng). Tyrosinase is active between a pH of 6.0 and 7.8. The optimal temperature

  • Effect Of Enzyme Concentration On Enzyme Reaction

    1046 Words  | 5 Pages

    experiments for week 5 and week 6 support each other in the further understanding of enzyme reactions. During week 5, the effects of a substrate and enzyme concentration on enzyme reaction rate was observed. Week 6, the effects of temperature and inhibitor on a reaction rate were monitored. For testing the effects of concentrations, we needed to use the table that was used in week 3, Cells. The 3 concentrations of enzymes were 0.5 ml, 1.0 ml, and 2.0 ml of turnip extract, while the substrate consisted

  • Catalase Lab

    1528 Words  | 7 Pages

    molecules produced per unit time). In order to do so, enzymes bind temporarily to one or more reactants, lowering the amount of activation energy needed and thus speed up the reaction. Most of the cells use an enzyme called catalase to reduce the amount of hydrogen peroxide, a toxic substance that could accumulate as a result of normal cell activity. The purpose of this experiment was to observe the effects that enzyme concentration and copper (II) sulphate would have on the rate of reaction. This

  • Lab Report Organic Compound Catecholase

    805 Words  | 4 Pages

    genetic mutation of potatoes, scientists can limit the amount of the substrate which causes less severe browning or no browning in potatoes. (Adams and Brown, 2007). The potato contains enzymes in which the catechol reacts with and it speeds the process up. If catecholase were left alone and without a catalyst (enzyme) the reaction would take much longer.

  • Baseline Peroxidase Reaction Lab Report

    1381 Words  | 6 Pages

    experiment B, we expected that as the enzyme concentration gets doubled, the absorbance values will increase faster than the baseline over time. The reason why is due to the double concentration of the enzyme that will allow for a better chance of binding to a substrate, which means that the reaction can occur faster. As for half the enzyme concentration, we expected that the absorbance values will increase but at a slower rate

  • Enzyme Lab Report

    1247 Words  | 5 Pages

    affect enzyme activity and its process. Enzymes are substances made in by an organism that serves as a catalyst and quickens the biochemical reaction to occur. When a catalyst is brought into the picture, it speeds up the time needed for said reaction to occur, making the enzyme a “helper” for reactions. Enzymes and substrates are closely related in the sense that both join together to help bring the end result of the reaction much faster. An environmental parameter, dealing with enzymes, is a particular

  • Enzymes: Biological Catalysts

    2446 Words  | 10 Pages

    An enzyme can be regarded as a catalyst for a biochemical reaction, or more simply, a biological catalyst. Its sole purpose is to increase the rate of a reaction, or speed up the reaction via provision of an alternative reactive pathway, which entails a lower activation energy. Enzymes participate in the reaction itself, enabling the occurrence of an alternate pathways of reaction, but they do not attain permanent changes to their structure or nature, and as a result remain unchanged preceding a

  • The Importance Of Enzyme Kinetics

    1522 Words  | 7 Pages

    • Enzyme Kinetics Enzyme kinetics Introduction It is the study of those reactions that are moderated by enzymes. In enzyme kinetics, the rate of reaction is measured and the effects of different conditions of the reaction are found out. Enzymes are protein in nature that moderate other molecules — the enzymes ' molecules . These target molecules bind to an enzyme 's activity site and are transformed into completed products through a series of steps known as enzymatic mechanism. These mechanisms

  • Catalase Lab Report

    560 Words  | 3 Pages

    Catalase, by definition, is the enzyme that decomposes potent H2O2 into harmless H2O and O2 (Sherwood 2016). Many factors such as the ones analyzed in this lab contribute to the overall rate of the chemical reaction. The first variable, enzyme concentration (Figure 2.), was expected to steadily increase the reaction velocity as more catalase was added. This is because Enzyme activity is generally greatest when substrate concentration is unlimiting (Worthington Biomedical Corporation). Since the substrate

  • How Enzymes Are Biological Catalysts

    566 Words  | 3 Pages

    Enzymes are biological catalysts, meaning that they speed up chemical processes in our bodies by lowering the activation energy required for them to take place. Like chemical catalysts, enzymes take place in the reaction, however, remain unchanged at the end. In humans, enzymes are involved in nearly all of the biological processes required for us to live. All known enzymes are proteins, hence are made up from chains of amino acids. They have an active site, which is the part of the molecule where

  • Enzyme Peroxidase Lab Report

    1281 Words  | 6 Pages

    Enzymes are biological catalysts that speed up the various chemical reactions that happen inside our cells. For the enzymes to function well, they need to be in an environment with a specific set of conditions, other than that it will lead to the denaturation of the enzyme or will render it completely inactive. There are a lot of factors that impact enzyme activities, but in this experiment we examined the effects of 4 different factors which are temperature, pH level, addition of the inhibitor and