Staining Essays

using selective and differential media. Materials and Methods: To begin this experiment, The Gram stain procedure will help identify a Gram-positive or Gram-negative bacteria. It begins with grabbing a clean glass slide and placing it on the glass staining rack. Flaming the inoculating loop sterile, use the DI water to create a bubble of DI water in the loop and placing it the center of the glass slide. Then flaming the unknown bacteria tube over the Bunsen burner as well as once again the inoculating

In experiment three, Gram staining was used to characterize bacteria, either gram positive or gram negative. The Bacillus slide was Gram positive for the results displaying that it had a strong affinity for crystal violet on applying the iodine. The Bacillus slide stained the color of violet because the thicker and richer the layer of the peptidoglycan walls, the more retention of the stain. While the Pseudomonas slide stained the color of red. The Pseudomonas slide was Gram negative due to the lack

bacteriologist who wanted to find a procedure to make bacteria that previously difficult to see under microscope, appear with ease. Gram became a pioneer in the microbiology field, because of his staining technique that he used on bacteria to increase their visibility in 1884. This staining method is called Gram Staining. The Gram stain is a differential stain where a decolorization step occurs due to two different basic stains. The Gram stain use a primary stain of crystal violet and secondary stain as safranin

The main purpose of this study is to identify the given unknown bacteria by gram staining and a series of biochemical test. There are many types of bacteria and they produce different enzymes. However, Some bacteria produces same enzymes, so specific tests were done to identify the particular enzymes. There are four main types of Gram-negative bacteria, which are Escherichia coli, Klebsiella pneumonia, Enterobacter aerogenes, and Salmonella typhimurium. Therefore, the unknown could be any of these

allowed to dry. Then, the smear must be heat fixed by exposing it to flame for few times until it got fixed. It is to prevent the cell from washing away during the staining and washing process. Then, it is air dried and followed by fixing it with flame from Bunsen burner. After fixing the smear, it must be stained using Gram staining solution, firstly crystal violet solution was flood onto it, and allowed for 1 minute, then wash off with tap water. Then, flood the slide with iodine solution for 1

The purpose of this lab report is to employ a myriad of skills, tools and, methods learned throughout this semester to perform the appropriate tests for the identification of the assigned unknown bacteria. Add more background information here!!! The most important tools and techniques used during this identification include aseptic technique, microscopic examination and, the use of selective and differential media. Aseptic technique is an important tool for microbiologists. It is imperative that

University, Clemson, SC], personal communication). Gram used tissue in his first attempt with staining (1). The staining of the organisms allowed for the viewing of structure and determining those components by the viewing of the color that appeared after staining the organism (2). The purpose of this experiment was to identify the organism as gram-positive or gram-negative. The first step in the gram staining procedure was to heat fix the smear to the slide to ensure that the organism did not rinse

Observations After conducting an experiment to find which plant, Tagetes erecta or Pelargonium graveolens inhibit bacterial growth. Results have shown that one plant, Pelargonium graveolens, prevented the growth of the bacteria, Staphylococcus, more efficiently and effectively than the Tagetes erecta. These results are evident as seen in the statistics provided above. The area around the Pelargonium graveolens sample just after day one was at 8 millimetres; day two increased to 9 millimetres and

Lab-experiment immunity and bacteria- How do they react? Research question: How does the bacteria Enterococcus Faecium SF 68 demonstrate resistance against the following antibiotics: Oxacillin, Climdacylin, Penicillin-G, Amikacin, Lincocymin, Erythromycin, Cephazolin, Mezlocillin ? Terminology used: Bacterium: Singular form of bacteria, one single individual. A bacterium an organism that possesses one single cell and is very adaptable to most environments. A bacterium contains only a single

Definition of microscope; the microscope is an instrument which is used for magnifying objects that are too small to see with the naked eye; such as cells, bacterias and other microorganisms (particles). It gives an enlarged image of the object. Definition of lens; a piece of glass, or another transparent material, with curved sides. Used for bending light rays (concentrating or dispersing) which passes through. The phenomenon that causes the light to bend when crossing the lens is called refraction

Unknown Lab Report Abiola Oyewumi March 16, 2015 Unknown #16 Abstract An experiment was conducted to determine which of the following unknown bacteria was in test tube number 16: Escherichia coli, Enterobacter aerogenes, Klebsiella pneumoniae, and Salmonella typhimurium. Biochemical tests were used to help identify the unknown bacteria. The Citrate test, Urease test, Triple Sugar Iron Agar test, Voges-Proskauer test, and Methyl Red test were the biochemical tests used in this experiment. The T-Streak

The Tragedy of Macbeth, by William Shakespeare, continuously uses the Blood and Staining motif. The use of this motif emphasizes Macbeths deplorable need to be safely thus, as a tyrant would when murdering those who have cared for him. It also emphasizes character, corruption and death. Macbeths need to be safely thus as a king is a psychological reaction from having murdering a king himself and knowing that rulers aren’t completely untouchable. It also can be a reaction from having known he had

Effect of different physical conditions on nitrogen fixing bacteria from rhizosphere Hypothesis: Rhizospheric Nitrogen fixing bacteria show optimal growth at PH: 6-7, Temperature: 30 °C and Salinity level: 0.005 – 0.010M NaCl INTRODUCTION: There is a huge bacterial diversity in rhizospheric soil. Gram-negative, non-sporulating baccilli which respond to root exudates are predominant in the rhizosphere (Pseudomonas, Agrobacterium). While Gram-positive

Every individual wishes to be healthy and disease free. Occasionally, the human health gets negatively affected due to pathogenic, disease causing microorganisms. In such cases, one takes antibiotics to cure themselves from this condition. Another mechanism to deal with this problem is vaccination which a prevention mechanism. Let us take a look at both these methods to fight diseases. Antibiotics are chemical substances produced by some organisms, and can kill or inhibit the growth of other microorganisms

An initial review of the crime scene photos indicates the possibility of latent bloodstains being present in certain areas. Looking closely at the snapshots suggests that certain areas have been cleaned. The door is clearly dirty in certain areas, yet a large portion of it is inexplicably clean and very white compared to the immediate surrounding areas of the door. On the floor, one can see something very similar with the metal plates. All of them are dirty and dusty to some extent – except the one

The Unknown Identification Lab was an experiment that provided the opportunity to apply all the tests that were learned in the semester of lab, to identify the two bacterias that remain unknown. Gram- staining and two other tests will be used to identify the unknowns. This experiment is crucial to the understanding of each test, and can benefit in the ability to identify the characteristics of specific bacteria. Having a clearer understanding of the bacteria can further the research of bacteria for

was hypothesized that the nostril microflora were gram-positive bacteria that belong to the genera Streptococcus, Staphylococcus and Propionibacteria. Results showed that the nostril microfloras were gram-positive (stained purple). However, gram staining was not enough to prove that the bacterium obtained from the nostril are Streptococcus, Staphylococcus, and Propionibacteria spp. The results do not fully support the hypothesis. Introduction All Fastidious microorganism rely on specific nutrients

particular compound help them to be identified by the biochemical tests. Gram’s stain was originally devised by histologist Hans Christian Gram in 1884. Gram-positive bacteria stain purple, while Gram-negative bacteria stain pink when subjected to Gram staining. Approximately 60-90% of the Gram-positive bacterial cell wall is made up of peptidoglycan and interwoven teichoic acid, while only

the organism appeared purple with cocci in clusters. The organism was also catalase positive which means that it produced enzyme catalase and bubbled when hydrogen peroxide was added to it. Three test were conducted based on the result of the gram staining procedure. Blood agar with a Novobiocin disk was chosen as well as DNase (DNA) and Mannitol Salts (MSA) agar. The Blood agar is a bright red, opaque plate and the streaking or the inoculation technique was a modified streaking for isolation with

Unknown #10 produced no identifiable macroscopic characteristics as a broth, so the first step was to Gram stain a loopful to determine the microscopic characteristics. Gram staining not only helped identify Unknown #10’s microscopic morphology but it also helped ensure the specimen was a pure culture—no other bacteria were visible when Unknown #10 was Gram stained and observed under the microscope. Unknown #10’s key microscopic morphology was that it was a very small, Gram negative bacillus. Though