ipl-logo

Dna Isolation Lab Report

1425 Words6 Pages

Results To have accurate results of the DNA isolated in this experiment, it must have expressed a purity value of 1.8. This can be observed by dividing the absorbance at 260 and 280. The DNA, in this case, was not very pure since the absorbance recorded was at 260 was 0.069 and the concentration was at 13.41 ug/uL. This number was lower than expected and could mean that there could have been some protein contamination when isolating the DNA. In terms of the characteristics of the SNPs, there are certain features that would be present in the results for the DNA ladder through the process of gel electrophoresis. The picture below shows the location where the isolated DNA was inserted in yellow. Figure 1: Image of Gel Electrophoresis under UV light for Observation of CDK3, TAS2R38, ADH/ADLH2, and D1S80 Genes Although the results were present but not as visible, here is a diagram below to show how the DNA ladder would have looked like once the isolated DNA ran through gel electrophoresis. Figure 2: Image of DNA Ladder In addition to this, there are examples of what could have been interpreted in the presence of the DNA ladder. It is evident that gel electrophoresis makes it possible to separate DNA fragments according to size …show more content…

The DNA isolated in the second column in red is heterozygous since it has two DNA bands visible. The DNA isolated in column five and column ten in blue indicates that these two individuals cannot be distinguished from each other according to the fingerprint. There could be a possibility that these two individuals are either twins or that they both have similar repeats, especially if only one STR is analyzed through the gel electrophoresis (Müller et al, 2013). All of these results helps to furthermore understand SNPs and shows how each one exhibits specific properties that are unique to each

More about Dna Isolation Lab Report

Open Document