Enzyme Absorbency Experiment

: The hypothesis was a failure because the absorbency was not consistently increasing or decreasing as the pH level increased. The absorbency kept decreasing and increasing at random pH levels. When the characteristics of the enzyme reaction were tested, test tube one was given ten drops of enzyme and ten drops of substrate as well as distilled water. The contents then turned from a clear to dark yellow when mixed with absorbency (A400) of 1.370, the highest absorbency out of all tests. The reactant that was lacking in the control reaction was the enzyme. It is important to include controls in an experiment in order to see how one substance can affect many other substances or organisms. The other controls that could have been performed were the

As the enzyme activity increased, the enzyme concentration also increased. However, increasing the concentration of a substrate does not promote enzyme activity. Considering what happens to the enzyme in an enzymatic reaction, the enzyme is still reusable. As the enzyme activity increases, the pH becomes constant. The enzyme is the most effective right before the pH becomes constant. The enzyme reaction fails to occur at very low and very high pH due to the causing of the enzyme to change shape. Since the shape determines the function, the enzyme will become unusable. The contents of the tubes changed mostly from clear to light yellow. The inhibitors that interfered with the enzyme action were pyrocatechol and tyrosinase, because they were the ones that caused the most absorbency. Increasing the inhibitor concentration affected the enzyme reaction because the most concentration there was, the higher the absorbency. In conclusion, the optimum temperature was twenty degrees Celsius, since it obtained the highest absorbency. As the pH levels increased, the data for absorbency was