LABORATORY REPORT Activity: Enzyme Activity Name: Natalie Banc Instructor: Elizabeth Kraske Date: 09.26.2016 Predictions 1. Sucrase will have the greatest activity at pH 6 2. Sucrase will have the greatest activity at 50 °C (122 °F) 3.
Abstract In this experiment it was examined whether the enzyme peroxidase will work fastest in a pH of 8.0. We placed the enzyme peroxidase in a reaction with guaiacol and hydrogen peroxide in four different pH solutions. Then recorded the absorbencies for each reaction until all substrates were used up, and calculated the initial reaction velocities for each. We found that the reaction in a pH 7.0 solution had the highest initial reaction velocity.
LABORATORY REPORT Activity: Enzyme Activity Name: Natalie Banc Instructor: Elizabeth Kraske Date: 09.22.2016 Predictions 1. Sucrase will have the greatest activity at pH 6 2. Sucrase will have the greatest activity at 50 °C (122 °F) 3. Sucrase activity increases with increasing sucrose concentration Materials and Methods Effect of pH on Enzyme Activity 1. Dependent Variable amount of product (glucose and fructose) produced 2.
The human body consists of enzymes which allow foods to be broken down and enable organisms to build chemical substances such as other proteins, carbohydrates and fats that are necessary for life. This experiment will be testing the enzymatic reactions with toothpicks. This experiment depicts the process when a substrate binds with an enzyme. However, only a specific substrate can attach to a specific enzyme. This allows the substrate to attach to the enzyme and then disconnect the enzyme overall causing the substrate to break in two.
Introduction: Enzymes are needed for survival in any living system and they control cellular reactions. Enzymes speed up chemical reactions by lowering the energy needed for molecules to begin reacting with each other. They do this by forming an enzyme-substrate complex that reduces energy that is required for a specific reaction to occur. Enzymes determine their functions by their shape and structure. Enzymes are made of amino acids, it 's made of anywhere from a hundred to a million amino acids, each they are bonded to other chemical bonds.
ST Report In the experiment, the problem was the contaminants that were affecting the quality of the water samples. To fix this issue, three scientists had to determine the contaminants that were present in the samples. One sample was from the school sink and the second sample was from an unknown source. The scientists conducted many tests to figure out what pollutants were present in the water.
In this lab, the optimal environment for an enzyme was observed in bacterial and fungal amylase. An enzyme is a substance produced by a living organism that is coded by proteins to catalyze chemical reactions throughout the body. Enzymes are what make the chemical reactions in living organisms possible. Enzymes act as catalysts to reactions, they lower the activation energy needed for reactions. However, enzymes require particular environments to function, and without proper conditions, enzymes
Enzymes are a type of catalyst that speed up reactions in the body. It allows the digestive system to speed up reactions that would otherwise have taken much longer and taken up more energy to execute, that could lead to a decrease of energy. Enzymes also break down poisonous chemicals made by the reactions of cells. It does so by separating toxins into harmless substances, a characteristic that makes enzymes vital for living organisms. For example, hydrogen peroxide (2H2O2) is a toxic byproduct of cellular respiration, but it is broken down by the enzymes into 2H20 and O2, two harmless substances, water and oxygen.
The lab performed was to test how different types of chemicals would affect rocks of similar sizes to measure the amount of mass lost due to weathering. The chemicals used for this experiment were solutions such as 50% vinegar, 10% vinegar, soda water, and regular tap water, which were used as a control. Using these samples, this experiment was conducted to showcase how different chemicals weather soil and minerals, along with how this weathering affects the topography of our environment. In order to understand the effects of chemical weathering in this experiment, it should be known that it occurs when a chemical substance such as acetic acid is introduced into an environment, causing the accelerated deterioration of soil and minerals found within the layers of the earth.
Enzymes. Lipids (Lipase) Lipase are secreted by the small intestine and pancreas. They digest complex lipids (fats) molecules into simpler, more soluble fatty acid and glycerol molecules. They exist of fats and oils and are made up of the elements carbon, hydrogen and oxygen and the most common type known of is the triglyceride.
The aims of this practical are to examine the effects of various substances on the activity of glycogen phosphorylase by the principles of allosteric control of the enzyme and reversible phosphorylation. These principles aim to reverse the effect glycogen phosphorylase has on the conversion of glycogen to glucose-1-phosphate, i.e. causing glycogen and glucose-1-phosphate to bind and release a phosphate. The amount of phosphate formed in this experiment is measured by the principles of a spectrometry reading at 660nm. [https://www.ncbi.nlm.nih.gov/books/NBK22354/]] Introduction: Glycogen is used in animals as a form of glucose that can be kept in storage in cells until there is a diminished amount of glucose in the body, which then glycogen
Compton scattering is the result of an incident photon with a high energy collides with a loosely bound outer shell electron. The outer shell electron is knocked out of the outer shell and this causes the initial photon to lose some of its energy. This results in a recoil electron and a scattered photon. When the collision happens the scattered photon changes direction and the recoil electron (Compton Electron) is the electron that was once on the outer shell is sent on its own path. The scattered photon’s energy can be calculated by subtracting the recoiled electron from the initial photon’s energy.
Biochemical tests are the tests used for the identification of bacterial species based on the differences in the biochemical activities of different bacteria. Bacterial physiology differs from one species to the other. These differences in carbohydrate metabolism, protein metabolism, fat metabolism, production of certain enzymes and ability to utilize a particular compound help them to be identified by the biochemical tests. Gram’s stain was originally devised by histologist Hans Christian Gram in 1884. Gram-positive bacteria stain purple, while Gram-negative bacteria stain pink when subjected to Gram staining.
MIDDLE EAST TECHNICAL UNIVERSITY FOOD ENGINEERING DEPARTMENT FDE 310 FOOD MATERIALS LABORATORY Experiment 5 Protein Determination Submitted to : Oya Nihan Uncu Section 2/Group 2 Merve Aylin Aydoğdu Büşra Karahan Özge Önder Elif Gökçen Sakar Burcu Başak INTRODUCTION Kjeldahl method was used in laboratory to determine the amount of protein in Feta Cheese. Determination of protein content in feta cheese, there are three steps which are digestion, neutralization and titration. More than 90 % of protein containing samples and over 60 % of all other nitrogen containing samples can be analyzed using this basic procedure.
KINETICS OF MULTISUBSTRATE REACTIONS Introduction Enzyme kinetics is the study of rate of biochemical reactions that are catalyzed by enzymes. In enzyme kinetics, the reaction rate is measured and the their effect is measured or investigated. Studying an enzyme kinetics in this way we can check the catalytic activity of enzyme, its major role in metabolism, and how its activity is determined. Enzymes are protein in nature and binds to substrates.
