Enzyme Lab Report

Materials and Methods: For the first part of the experiment is to measure the absorption of different diluted solutions of enzymatic extract. A spectrophotometer and three Erlenmeyer flasks are required. Set the spectrophotometer to 540nm. Number the flasks from one to three and add 30 milliliters of enzyme extract the flask marked one on it. It is best to split up the work into three groups. Next, add 20 milliliters to the flasks marked two and three. Pipette 10 milliliters of enzyme extract from flask one to flask two making a 1:3 solution of water and enzymatic solution in flask 2. Next, Pipette 10 milliners of flask twos 1:3 solution into flask three creating a 1:9 dilution. Remove 10 milliliters of diluted enzyme from flask three and

Each group using one cuvette to prepare a blank for each of their solutions and pipette out 1.5 millimeters of solution from their assigned flask into their own cuvette and adding three milliliters of water to the cuvette then adding three drops of lugols iodine into each cuvette creating the blank. Next, zero the spectrophotometer with the prepared blank. Add three drops of the lugols iodine to six empty test tubes to measure the times of reaction. When ready to time, add 20 milliliters of starch to the groups flask who has the 1:9 dilution. Each group can begin timing after transferring three milliliters of the enzyme extract to the test tube containing the iodine and that mixture pipetted it into a cuvette. After recording the initial absorption, blank the spectrophotometer with the prepared blank and repeat the process of measuring and blanking every thirty seconds for six

First, add three milliliters of water as well as three drops of lugols iodine into a test tube and the pipette 2.5 milliliters to be the blank in this experiment. Have four flasks and number them one through four and add 22 milliliters of water and two milliliters of starch solution. Acquire four test tubes and pipette one milliliter into each test tube and then put them on ice to chill.

Add three drops of lugols iodine to eight test tubes. place flask numbered one as well as the test tube numbered one in an ice bath for five minutes. Have the flask and tube numbered two sit and be at room temperature. Add the contents of test tube two to flask two and mix. Next, pipette three milliliters of flask two into a test tube with lugols iodine present in the tube and then transfer it into a cuvette and measure the absorbance. Repeat this process every thirty seconds while blanking the spectrophotometer for a total of seven minutes. Blank the machine after