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Redox Reaction Lab Report

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Recommended: Introduction To Enzyme

Introduction:
Enzymes are proteins that function as catalysts, meaning that they increase the speed of a reaction without being changed themselves. The enzyme has two main jobs in a reaction that cause the reaction to increase. The first job is to bring substrates (the substances that the enzyme will be reacting on that bind to the active site in the beginning a reaction) together in an orderly fashion so that they can interact during the reaction. It’s second job is to decrease the energy needed for a reaction to take place. These tasks can be completed more efficiently in specific temperatures or with specific pH levels. The speed of a reaction can increase as temperature increases. This is because heat is a representation of kinetic energy: …show more content…

This reaction occurs through both oxidation and reduction. Oxidation is the process of a compound losing electrons by binding with oxygen. Reduction occurs when a separate compound accepts these electrons. In this particular experiment, the enzyme peroxidase, which is specified to break down hydrogen peroxide, will be used to catalyze the redox reaction. The substrates will be reduced guaiacol and hydrogen peroxide (H2O2). This reaction will produce oxidized guaiacol, oxygen gas, and water. In the oxidation of guaiacol it will change colors, this is what shows the reaction has occurred. To monitor the reaction, a spectrophotometer will be used that will measure the absorbance of light. If the substance is a darker color, it will absorb more light, and if it is lighter it will absorb less light. In addition, the enzyme and substrate will be tested with different levels of pH and different temperatures in order to demonstrate if the reaction will improve or not based on these …show more content…

This test tube lacked hydrogen peroxide in order to keep the reaction from occuring. In two test tubes labeled “Substrate”, we mixed 0.2 ml of hydrogen peroxide with 0.1 ml guaiacol and 4.7 ml of distilled water. We also labeled two test tubes “enzyme” using a glass marking pen and filled them with 1.0 ml of turnip extract and 4.0 ml of distilled water. After the test tubes were prepared, we put the blank test tube into a cuvette and put it into the spectrophotometer in order to zero it out. While one group member set the spectrophotometer to zero, another mixed one enzyme test tube with one substrate tube and observed a change in it’s color. After we set the spectrophotometer to zero, we mixed the second enzyme with the second substrate and promptly poured it into a clean cuvette to then be put in the spectrophotometer and record the absorbance at zero seconds, and again every thirty second for three

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Enzymes are a form of protein that lowers activation energy and speeds up reactions as a catalyst. They are made by the stringing together of an abundant amount of amino acids and folded into a specific shape for chemical reactions. Turnip Peroxidase is the enzyme used in this lab and is derived from the vegetable. Enzymes are not used up or permanently altered by their environment Peroxidases are found in a range of organisms and function to break down alcohol (H2O2) and creates byproducts of oxygen and water. In this experiment, the reducing agent guaiacol is added with the substrate, hydrogen peroxide, to create water and oxygen.

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After the 15 minutes, each pair was removed from their assigned temperature and mixed with its partner. The mixed solution was then poured into the appropriate tube and placed in the spectrophotometer for 120 seconds. As peroxidase was broken down a brown color appears and is measured by the spectrophotometer. The absorbance readings were recorded every 20

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1 “substrate” and another “ enzyme.” Instead of using the distilled water, this time you are going to use different pH buffer in the enzyme test tube. In the substrate tube, add 7 mL of distilled water, 0.3 mL of hydrogen peroxide, and 0.2 mL of guaiacol for a total volume of 7.5 mL. For the enzyme tube, instead of distilled water add the pH solution (3) and 1.5 mL of peroxidase which equals a total volume of 7.5 mL. Use the dH2O syringe for our pH solution. To clean the syringe, flush it by drawing 6 mL of distilled water.

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While conducting this experiment readings were took every 15 seconds for 5 minutes which equals to a total of 20 readings. This experiment had four different trials, a base line trail, a pH 4 trail, a pH of 7 trail, and a pH of 8 trail. Then the results were recorded. The enzyme peroxidase was taken from the inside of a turnip (2g), then blended with 150ml of deionized water, and then poured through a coffee filter into a beaker for a smooth solution. Secondly the colorimeter had to be calibrated by placing a clean cuvette only containing the enzyme solution.

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