The sugar used in this experiment was created by mixing ten milliliters of starch,glucose, and regular countertop sugar. This was transferred into the joint water bottle tunnel using a funnel. Both ends of the tunnel were sealed shut and each measurements were taken every three minutes and final measurements were taken after 21 minutes. The results were
The percentage of glucose was recorded for each sample. Next, the test tubes were carefully cleaned with soap and water. Then five millilitres of sample “A” was placed in the test tube labeled “A”. This was then repeated with the next three samples. 20 drops of Biuret reagent were then added to each test tube.
Sucrase activity increases with increasing sucrose concentration Materials and Methods Effect of pH on Enzyme Activity 1. Dependent Variable amount of product (glucose and fructose) produced 2. Independent Variable pH 3. Controlled Variables temperature, amount of substrate (sucrose) present, sucrase + sucrose incubation time Effect of Temperature on Enzyme Activity 1.
B-galactosidase breaks down the disaccharide lactose into simple sugars glucose and galactose. However, glucose is a colorless compound hence it has to be substituted with a compound that is detectable by a visible color change. Hence,
The result for Enterobacter aerogenes is A+G+, and for unknown #36 is A+/-G+, this test while not identical is not completely different and shows possibility that the test differed because of human error during lab such as not sampling a successful amount of bacteria in the test tubes. Another human error is results not being read correctly. This is possible as the results are based on color, which can sometimes be interpreted differently based on lighting, concentration or experience of the person reading the results. These same ideas may serve as an explanation for the difference in test results for lactose and glucose fermentation experiments. The last test that did not concur was pigmentation.
Bio: The following artifacts are from the Biology classes that I have taken at Central Washington University (CWU): The Lactose and lactase lab report is an investigation on how gastric acidity and temperature affect the effectiveness of a commercially available lactase product. The genetics exam demonstrates my knowledge about inheritance as the result of sexual reproduction. The photosynthesis and cellular respiration document demonstrates my understanding about the chemical processes that take place in plant and animal cells to create Adenosine triphosphate (ATP). The ecosystems, population, and selection document is a compilation of notes that describe the source and flow of energy, the factors responsible for speciation, and selection
The Effect of Sugar Concentration on CO2 Production by Cellular Respiration in Yeast Introduction In this lab, our main focus was to find how sugar concentration affect yeast respiration rates. This was to simulate the process of cellular respiration. Cellular respiration is the process that cells use to transfer energy from the organic molecules in food to ATP (Adenosine Tri-Phosphate). Glucose, CO2, and yeast (used as a catalyst in this experiment) are a few of the many vital components that contribute to cellular respiration.
This assignment is centered around current hot nutrition topics in the area of carbohydrates in the diet. You must THOROUGHLY answer these questions, and spell and grammar check before submitting. Remember that your assignment will be run through plagiarism detection software. 1. (4pts)
INTRODUCTION Metabolic syndrome (syndrome X, insulin resistance syndrome) consists of constellation of metabolic abnormalities which include central obesity, hyperglycemia plus insulin resistance, high triglycerides plus low HDL cholesterol, and hypertension.1 NCEP:ATP III 2001 Criteria for the Metabolic Syndrome2 :- Three or more of the following: . Central obesity: Waist circumference ≥102 cm in males or ≥ 88 cm in females .Hypertriglyceridemia: triglycerides ≥ 150mg/dl .Low HDL cholesterol < 40 mg/dL (males) or < 50 mg/dL (females) .Hypertension : blood pressure ≥ 130mm systolic or ≥85mm diastolic .Fasting
Fermentation uses more glucose because the process of fermentation is much less efficient than cellular respiration in terms of energy production per molecule of glucose used. The open flask (control) and the closed
Response surface methodology is a group of techniques that are used to study the relations between one or measured dependent factors (responses) and several input (independent) factors [27]. The effect of concentration of the three selected variables, ammonium sulphate, yeast extract and 1,2 propylene glycol was studied by this method. The concentration ranges selected for the three factors are listed in (Table 4). All other factors (glucose 10 g/L, glycine 0.2%, temperature 28 °C, initial pH of medium 7.0, agitation rate 200 rpm, inoculum volume 2%) were kept constant. To calculate optimum values of selected three factors, a set of 20 experiments was generated using a 23 full factorial CCD, with six replicates at the centre point, was employed to fit a second order polynomial model.
The results of the phenol-sulfuric acid analysis conducted in this experiment suggest that the data acquired was relatively precise but inaccurate with respect to the given carbohydrate concentrations of the soda and Gatorade samples. Using a standard curve generated from a glucose solution with a known concentration, the carbohydrate concentration of the samples was determined (in terms of glucose) and a low coefficient of variation was calculated. However, a high percent relative error was apparent in the analysis of both samples. This may have been due to the fact that the analysis was conducted assuming glucose was the carbohydrate of interest, while, in fact, a significant portion of the monosaccharides would have existed as fructose (a
PROCESSING OF BISCUITS Mixing: Creaming- The ghee, sugar and vanilla flavor is churned so that flavor may get adhered to the fine droplets of fat. Mixing time is of two minutes.
Introduction: Lactose intolerance is a condition concerned with the inability of the small intestine to produce sufficient quantities of the lactase enzyme in order to digest lactose (1). Approximately two thirds of the world’s population is affected by this inability and it is often one of the most prevalent gastrointestinal disorders diagnosed by primary care physicians (2, 3). When a person suffering from lactase deficiency consumes lactose in their food, their body is unable to process and absorb the lactose, and so it travels directly to the colon (4). Once it has reached the colon, the undigested lactose interacts with the normal colonic bacteria, presenting the signs and symptoms of lactose maldigestion (4).
Blood glucose test or monitoring is a quantitative test. This test used to measure the amount of sugar in the blood. This test is usually done by an individual who suffers from diabetes to determine and monitor their daily blood glucose level. This test is a good way in order to determine the patient’s type of treatment and also determine the level of blood glucose either too high or low. A research done by the Diabetes Control and Complications Trial done in 1993 showed that blood glucose monitoring led to lesser complications in disease.