Analysis of Major Unknown 3C Summary: A series of tests was done with Microbe 3C. A Gram stain was done and the sample was gram positive. A streak plate was completed revealing that the microbe was clear/white in color, margin was entire, shape was circular, elevation was convex/raised, and the opacity was translucent. The last test done was the sucrose test. It starts orange and turns yellow positive for microbe 3C Klebsiella oxytoca (Professor Brady, Personal Communication). Procedure 1 Gram stain: Purpose/ Introduction: The Gram stain reveals whether a microbe is gram positive or gram negative. It also reveals the shape and arrangement of the microbe (Professor Brady, Personal Communication). Of the forty-three possible microbes the following are gram positive: Bacillus cereus, Bacillus circulans, Bacillus coagulans, Bacillus megaterium, Bacillus subtilis, …show more content…
The following was eliminated because they are gram positive: Bacillus cereus, Bacillus circulans, Bacillus coagulans, Bacillus megaterium, Bacillus subtilis, Bacillus thuringiensis, Brevibacillus brevis, Geobacillus stereothermophilus, Lysinibacillus sphaericus, Clostridium perfringens, Corynebacterium pseudodiphtheriticum, Listeria monocytogenes, Lactobacillus casei, Enterococcus faecalis (Streptococcus), Streptococcus lactis (Lactococcus), Streptococcus mutans, Streptococcus pneumonia, Micrococcus (Kocuria) roseus, Sarcina lutea (Micrococcus luteus), Sporosarcina ureae, Staphylococcus aureus, Staphylococcus epidermidis, Staphylococcus saprophyticus, and Rhodococcus rhodochorous. The remaining microbes are gram negative: Alcaligenes faecalis, Rhizobium radiobacter, Salmonella typi, Serratia marcescens, Shigella flexneri, Yersina pseudotuberculosis, Erwina persicinus, Serratia liquifacens, Halobacter salinarium, Enterobacter aerogenes, Eschirichia coli, Flavobacter capsulatum,