ipl-logo

Restriction Enzyme Lab Report

450 Words2 Pages

When scientist began studying the way that bacterial protect themselves from different types of viral and from bacteriophages, they realize that inside bacterial cells are these special digesting proteins and special digestive enzymes known restriction enzymes, these restriction enzyme are able to a cut or cleave the viral DNA molecule into many different pieces thereby destroying the viral DNA and deactivating the viral DNA. There are many different types of restriction enzymes that exist in nature and each restriction enzyme cleaves a longer DNA molecule at a specific location on that double stranded DNA molecule. Different types of restriction enzyme also recognize a different sequences in a DNA and and it will cut in different way. Restriction enzyme cuts the DNA …show more content…

One example of a restriction enzyme is EcoR5 cuts DNA somewhere along the sugar phosphate backbone. It can only make the cut if a certain sequence is present within the DNA. Eco R5 will only cut DNA if it see’s the sequence GATATC. Eco R5 makes a cut between the TA and then the TA in the complementary strand. Biologist are interested in knowing how big the pieces are that resulted from restriction enzyme cut. One conventional way of describing the length of DNA is by counting the base pairs. Once scientist actually form that recombinant DNA molecule they will need many copies of the enzyme in order to conduct many results, so what scientist do is they use plasmid. The bacterial cells has smaller circular DNA known as plasmid. Scientist can take a specific plasmid from a specific location and create a recombinant plasmid. The recombinant plasmid can be inserted into a bacterial cell and that bacterial cell will divide many times via binary fission and produce many identical copies of the recombinant plasmids and ultimately we can take these bacteria cell and work with

Show More
Related
735 Words | 3 Pages

How could a single nucleotide change on the plasmid alter the fragment pattern observed in the gel? (3 points) When a single nucleotide is change on the on the plasmid, it will make the restriction enzyme not to recognize the fragment pattern and also all the numbers at each site will be altered or this action could make the fragment not to be incised at the expected site, which could make a larger fragment to travels a short interval on the gel with less fragments than expected. It could also make it to be incised at more places than that originally expected and the result will be increased, smaller fragments that move further across the gel. There may also be no change in the banding pattern.

Read More
1419 Words | 6 Pages

The plasmid and the gene are both cut using restriction enzymes. To incorporate the gene into the plasmid, both the plasmid and gen stuck together by a bacterial enzyme called ligase. The plasmid with the foreign gene can then be inserted into the bacteria One specific plasmid is pUB110 which is circular whose host is Bacillus subtilis. It has a plasmid size 2.3 kpb and copy number between 20 - 50. We can selectively grow bacteria that contain this plasmid by using selective and differential media.

Read More
984 Words | 4 Pages

In addition, the bacteria need arabinose that help activate the protein that allows to glow green in the transformed bacteria cells. To let

Read More
681 Words | 3 Pages

Each restriction enzyme distinguishes one or few

Read More
1120 Words | 5 Pages

After setting aside those two -RNase tubes, 4 μL of RNase was pipetted into each of the original aqueous tubes. Then, 6 μL from the aqueous tubes, which now contained RNase, were transferred into two new, clean and labeled 1.5ml tubes; moreover, these two tubes were labeled as +RNase in addition phenotype. At this point we had three sets of tube, each set containing one of each phenotype; moreover, one set was labeled -RNase, another was labeled +RNase and lastly, a set labeled aqueous. Finally, in two new, clean, and labeled 1.5 mL tube, using a pipettor, 95 μL of sterile molecular grade water and 5 μL of each RNAS treated DNA was added; these were labeled as 1:20 dilution in addition to the phenotype (CMMB,

Read More
1057 Words | 5 Pages

It is through the process of heat shock, we were able to genetically transform the bacteria

Read More
250 Words | 1 Pages

The human body consists of enzymes which allow foods to be broken down and enable organisms to build chemical substances such as other proteins, carbohydrates and fats that are necessary for life. This experiment will be testing the enzymatic reactions with toothpicks. This experiment depicts the process when a substrate binds with an enzyme. However, only a specific substrate can attach to a specific enzyme. This allows the substrate to attach to the enzyme and then disconnect the enzyme overall causing the substrate to break in two.

Read More
901 Words | 4 Pages

The enzymeʼs have an active site that allows only certain substances to bind, they do this by having an enzyme and substrate that fit together perfectly. If the enzyme shape is changed then the binding

Read More
1425 Words | 6 Pages

It is evident that gel electrophoresis makes it possible to separate DNA fragments according to size

Read More
2777 Words | 12 Pages

Restriction enzyme basically are enzymes that cut DNA somewhere along its sugar phosphate backbone (Cheng 2015). They are naturally found in bacteria. Bacteria can be infected with viruses and as a way of protecting themselves against viral infections, what a lot of bacteria can do is that they can make these restriction enzymes. And the purpose of making them is to chop up any viral DNA that tries to infect the bacterial cell. The most important thing about restriction enzymes is that they cut the DNA at a particular and specific location, not random sequences (Nassan,

Read More
482 Words | 2 Pages

The enzyme will recognize and cut along short, specific sequences of base pairs, called restriction sites, which are typically four to eight base pairs long. The restriction sites are palindromic, with nucleotide sequences that read the same on the forward strand as it does on the reverse strand when both are read in the same orientation. Restriction enzymes are naturally occurring in bacteria, and they aid in the protecting the bacteria from from bacteriophages viruses. Bacteriophages attack bacteria by inserting their genetic material into the cell which leads to an infection. However, some bacteria, such as E. coli, have restriction enzymes that can destroy foreign genetic material by digesting and ultimately inactivating the genes.

Read More
191 Words | 1 Pages

Enzymes are a type of globular protein that function within the human body as catalysts for other biochemical reactions. Catalysts are agents within a specific reaction that are able to quicken the reaction process without being altered themselves. Substances that these enzyme catalysts are able to convert into products are labelled as substrates. In order for the enzyme to catalyse the specific biochemical reaction, the substrate must bind to a special region on the surface of the globular enzyme (known as the active site). However, the shape and chemical properties of the active site and the substrate must match each other in order for the reaction to proceed.

Read More
1093 Words | 5 Pages

In the lac operon of E.coli, lactose induces the synthesis of the enzyme, beta-galactosidase. This enzyme codes for the Lac Z gene and thus, when synthesized properly with no other affecting factors, would break down ONPG. If lactose is not present in the enzyme, then the Lac I gene would continue to block transcription of the genes are also not synthesized. The primary regulator of the lac operon is a negative control element known as the lac regulatory protein which acts as a repressor when binded to a gene, turning the expression of the gene off. The repressor is a regulatory protein that binds to the operator and blocks transcription of the genes of an operon.

Read More
875 Words | 4 Pages

Usually, the microbial enzymes have various potential uses in industries and medicine. The microbial enzymes are also more reliable than plant and animal enzymes as they are more stable and active. Also the microorganisms demonstrate an alternative source of enzymes because they can be cultured in large quantities in a short time by fermentation and owing to their biochemical diversity and susceptibility to gene manipulation. Industries are looking for new microbial strains in order to produce different enzymes to fulfil the current enzyme

Read More
1251 Words | 6 Pages

Finally, the amplified DNA regions are compare using a gel. DNA Profiling

Read More

More about Restriction Enzyme Lab Report

Related Topics

Open Document