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Restriction Enzyme Lab Report

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When scientist began studying the way that bacterial protect themselves from different types of viral and from bacteriophages, they realize that inside bacterial cells are these special digesting proteins and special digestive enzymes known restriction enzymes, these restriction enzyme are able to a cut or cleave the viral DNA molecule into many different pieces thereby destroying the viral DNA and deactivating the viral DNA. There are many different types of restriction enzymes that exist in nature and each restriction enzyme cleaves a longer DNA molecule at a specific location on that double stranded DNA molecule. Different types of restriction enzyme also recognize a different sequences in a DNA and and it will cut in different way. Restriction enzyme cuts the DNA …show more content…

One example of a restriction enzyme is EcoR5 cuts DNA somewhere along the sugar phosphate backbone. It can only make the cut if a certain sequence is present within the DNA. Eco R5 will only cut DNA if it see’s the sequence GATATC. Eco R5 makes a cut between the TA and then the TA in the complementary strand. Biologist are interested in knowing how big the pieces are that resulted from restriction enzyme cut. One conventional way of describing the length of DNA is by counting the base pairs. Once scientist actually form that recombinant DNA molecule they will need many copies of the enzyme in order to conduct many results, so what scientist do is they use plasmid. The bacterial cells has smaller circular DNA known as plasmid. Scientist can take a specific plasmid from a specific location and create a recombinant plasmid. The recombinant plasmid can be inserted into a bacterial cell and that bacterial cell will divide many times via binary fission and produce many identical copies of the recombinant plasmids and ultimately we can take these bacteria cell and work with

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