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Gene Mapping Of Fungus Sordaria Fimicola Using Tetrad A

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Gene Mapping of Fungus Sordaria fimicola Using Tetrad Analysis
Devan Endejan

Biology 220
October 30, 2015

INTRODUCTION
This lab is used to exhibit gene linkage and mapping with tetrad analysis. Gene mapping is when a method is used to determine how close two genes are, the closer the genes are the more likely those genes are inherited together. For the lab we used a fungus Sordaria fimicola, because this particular fungus produces spores that can be easily observed with tetrad analysis. Tetrad analysis is a method used for fungi to show linked relationships by evaluating the products from meiotic division. The asci of S. fimicola stay complete with tetrad analysis which makes the spores easily observed for genetic cross-over. This lab …show more content…

Eight ascospores are produced form one zygote, which are lined up in an ascus. The ascospores are haploid and all the alleles are phenotypically shown. The fungus Sordaris when normal has a spore color that is black. In this lab we will be using gray and tan spores to mate. The allele form for the gray spore gene is g+ which is the wild type allele, and the other is g, which is the mutant allele. The tan spore gene has a t+ for the wild type allele, and the mutant allele t. The black ascospores genotype is g+t+, gray will have the genotype g t+ and the tan will be g+t, and colorless genotype will be g …show more content…

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