Worm Dna Lab Report

Nematode Worm Gene Amplification

Jennifer White

Biology 1050 - Section 003

Worm DNA Extraction, Analysis and Comparison with Human DNA

9/25/2017

Introduction:

In this lab experiment students replicated the exercises as dictated in the lab manual with few exceptions as prescribed by the professor. The genetic material of three varieties of C. elegans worms were examined as a model for genetic human disease in this study via Polymerase Chain Reaction technique. An agarose medium and gel electrophoresis set up was provided students along with materials for DNA amplification. The results were recorded by photograph and in the lab manual.

Mutations occur as a natural product of evolution whether good or bad. As described by Professor Lundquist during his tenure at Kansas University, humans have evolved from a long line of cells including those that may well have included the

The full citation can be found at the end of the report under the heading “Citations.”

Part 1: Isolation of Worm DNA: Students were asked to use a two-step lysis procedure involving a lysis buffer, centrifuge along with freezing and reheating to procure the worm DNA.2

Part 2: Students practiced using microliter pipettes p20 and p200 to obtain genetic material and fill appropriate tubes for the genetic material to be processed through the minicentrifuge.2

Part 3: Students used Polymerase Chain Reaction to replicate and therefore amplify the worm DNA. This procedure would allow the base pairs to show up next to a standard ladder of genetic material provided by the professor. The lab was set up with tubes containing PCR-Ready-To-Go beads which contained the nucleotides needed to build DNA copies, DNA polymerase and a lysis buffer which would help to ensure the stability of the reaction used to extract the worm DNA from its nucleus.2