The Effect of Substrate Concentration on the Temperature of the Enzymatic Reaction with Catalase Background: In this experiment, the effect of substrate concentration on the temperature of the enzymatic reaction with catalase will be tested. Enzymes are a class of proteins that serve as a catalyst, meaning that it lowers the activation energy required for a reaction without being consumed or altering the end products. Activation energy is the minimum amount energy required to begin a reaction. Enzymes contain an active site at their R group, which provides favorable conditions for the reaction to occur. In order for an enzyme to act on a substrate, the substrate has to enter the active site with the minimum rate of energy for the reaction to occur. …show more content…
The mean difference in temperature in the enzymatic reaction with 4 mL H2O2 was 5.20. The mean difference in temperature in the enzymatic reaction with 6 mL H2O2 was 5.60. This data demonstrates that the temperatures increased as substrate concentration increased. The means of the temperature differences of the three groups are displayed in Graph 1. The standard deviation of temperature difference in the enzymatic reaction with 2 mL H2O2 was 0.125. The standard deviation of temperature difference in the enzymatic reaction with 4 mL H2O2 was 0.497. The standard deviation of temperature difference in the enzymatic reaction with 6 mL H2O2 was 0.294. These standard deviations indicate that the data was distributed relatively around the mean. The variability of temperature difference in the enzymatic reaction with 2 mL H2O2 was 0.038. The variability of temperature difference in the enzymatic reaction with 4 mL H2O2 was 0.096. The variability of temperature difference in the enzymatic reaction with 6 mL H2O2 was 0.053. Since the variabilities are below 0.2000, this indicates that the data was reliable because there was little