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Effectors on helicobacter pylori
Effectors on helicobacter pylori
Effectors on helicobacter pylori
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More handpicked essays just for you.
Effectors on helicobacter pylori
Effectors on helicobacter pylori
Effectors on helicobacter pylori
Don’t take our word for it - see why 10 million students trust us with their essay needs.
OBJECTIVES To solicit for aid to help study the biological importance of bacillus megaterium and to determine whether bacillus megaterium is susceptible or receptive to antibiotics by investigating the characteristics of the genus species and also. SUMMARY The purpose of this experiment is to be able to identify Bacillus megaterium using 16S PCR & Sequencing and also determine whether bacillus megaterium is susceptible or receptive to antibiotics using gram stain. Being able to determine whether bacillus megaterium is susceptible or receptive to antibiotics is critical to medical advancement and biotechnological enhancements.
According to the series of test that my group ran for our unknown specimen, we had a match with the bacteria known as Alcaligenes Faecalis. This bacterium belongs to one of the major group of gram-negative bacteria (Phylum Proteobacteria). Alcaligenes Faecalis (Genus, species) is a rod shaped (bacillus), 0.5-1.2 x 1.0-3.0 µm, round with scalloped margin (colony configuration growth), motile (with one to nine peritrichous flagella), gram-negative, non-fermentative bacteria, obligate aerobic, having oxygen as the principal terminal electron acceptor in the electron transport chain (ETC). We consider we have a match with the species Alcaligenes Faecalis because of the following reasons: Fermentation tests performed (Durham sugars) were negative, which indicate that our bacteria use a different metabolic means for growth (non-fermentative gram-negative bacteria).
Unknown #10 produced no identifiable macroscopic characteristics as a broth, so the first step was to Gram stain a loopful to determine the microscopic characteristics. Gram staining not only helped identify Unknown #10’s microscopic morphology but it also helped ensure the specimen was a pure culture—no other bacteria were visible when Unknown #10 was Gram stained and observed under the microscope. Unknown #10’s key microscopic morphology was that it was a very small, Gram negative bacillus. Though bacilli can possibly form endospores, no empty white centers were visible which suggested that Unknown #10 was not an endospore forming bacteria. No quick endospore stain was performed to validate this assumption since only one assigned organism was endospore forming and unlike Unknown #10, that organism was Gram positive.
The purpose of this lab report is to employ a myriad of skills, tools and, methods learned throughout this semester to perform the appropriate tests for the identification of the assigned unknown bacteria. Add more background information here!!! The most important tools and techniques used during this identification include aseptic technique, microscopic examination and, the use of selective and differential media. Aseptic technique is an important tool for microbiologists. It is imperative that aseptic technique is maintained throughout the length of any test to avoid any cross-contamination that may lead to inaccurate results.
The unknown bacteria was then tested on multiple selective and differential media. Growth was present on the MacConkey Agar and the colonies were the same color as the plate, which told me my bacteria was gram negative and did not ferment lactose. There was no growth on the Mannitol Salt Agar, and this told me the unknown was not salt tolerant and did not
In 1981,Barry Marshall had a theory for the cause of duodenal and gastric ulcers. Scientist didn 't believed him, so he decided to drink a bacterial cocktail to prove his theory was right. Although, he became very sick and severed from nausea, Marshall took an endoscopy which revealed inflammatory signs of gastritis and the presence of H. pylori. Marshall results proved that H.
To limit the amount of errors or contamination in any procedure lab safety rules, gloves, and the aseptic technique was strictly enforced throughout the experiment. The first step to identifying the unknown bacterium was the Streak Plate Method. This method is used to isolate a pure culture from a mixed culture. Also, this method included streaking a tryptic soy agar (TSA) plate into four quadrants, and later incubating the plate for 24 hours.
These microorganisms are used to teach us how multicellular organisms came to be and how they can survive today. These small, microscopic organisms are so unique that the identification of them is paramount in the advancements of science. Knowing the chemical makeup, the shape, and the biochemical processes is important in identifying these organisms to understand how they survive and where. A number of tests can be ran on an unknown bacteria to determine their ideal
By what mechanism do the authors propose that the mcr-1 gene confers colistin resistance, and what evidence do they use to support this assertion? The protein sequence of mcr-1 showed its similarity to the polymyxin-producing bacterium, Paenibacillus spp., which showed the possibility of gene transfer occurring. The mcr-1 gene enables protection from polymyxin. The mechanism that the authors proposed on how the mcr-1 gene confers colistin resistance is that mcr-1 causes a modification in lipid A, present in the lipopolysaccharides of most bacteria, which leads to lessened polymyxin affinity.
Being able to determining the identity of a bacterium is essential as treatments for different types of bacteria vary greatly. Without correct and timely identification of a bacterium, a patient’s condition is likely to worsen and the probability of death rises due to inaccurate treatment. The identification of bacteria is also crucial in the prevention of bacterial diseases. Identification of bacteria also aids the selection of antibiotics used against them. This assists in the reduction of the overuse and misuse of antibiotics which aids bacterial resistance to antibiotics.
The bacterial DNA is circular inside of an E. coli bacterium. E coli. is most known for being found in the intestine of humans and animals but it can also be found in other places such as food
Introduction: Microbiology is the study of the microbial world, which is composed of microorganisms that are too tiny to be seen with a naked eye. This project demonstrates the significance of correctly identifying unknown organisms with methods and techniques acquired in the laboratory as they can make or break one's ability to accurately isolate and identify various species. Studying microbes enables oneself to have an insight on not just their structure or how they function, but how they have an impact on organisms and their environment around them as they can be found nearly anywhere. The importance of the process of identifying unknown bacteria vary from determining the causative agent of a disease, to testing if a specific food or fluid is safe to consume, and simply knowing what is present
Clostridium perfringens Clostridium perfringens is a bacteria which are Gram-positive, endospore-forming anaerobes, that appear to be rod-shaped. They lack flagella, but they can still migrate across surfaces using a type of gliding motility that involves the formation of filaments of bacteria lined up in an end-to-end conformation. Clostridium perfringens bacteria is most commonly found in soil, and it the intestinal tracts of humans and animals. (1,2) C. perfringens most commonly causes food poisoning, and various types of gangrene, infections from this bacteria can cause necrosis, bacteremia, and emphysematous cholecystitis. Back before the 1890’s Clostridium perfringens used to be known as Clostridium welchii.
Most people have heard of the “Black Death,” or the “Great Plague” but only because it was an epidemic. Taking a closer look at the Great Plague enables focusing on the factors that helped it spread not just the aftermath. It is important to have basic knowledge and understanding of the plague in order to be able to focus in on the factors involved in allowing it to prevail. It is believed that migration and trade had a substantial amount of influence on the prevalence and the spreading of the plague. The prevalence also allowed for a dramatic change within the European countries socially and economically.
Escherichia Coli 0157: H7 This paper will specialize on a specific type of bacterial foodborne illness caused by the bacteria Escherichia Coli. E. coli was discovered by Theodore von Escherich in 1885. E.coli is a natural found bacteria that lies throughout the intestinal tract of warm blooded animals and comes in many forms only one of which is deadly. This form is E. coli 0157:H7 which can be caused by direct exposure to fecal matter to kill this rouge