INTRODUCTION Two organisms that cannot produce gas when grown separately but can produce gas when grown in association, that is termed synergism. S.aureus and E.coli are also referred as synergistic pair. A sucrose is the sugar that some organisms can utilize because it is having the enzymes that break down the compound. Acid or gas is produced when the organisms are grown in association. To determine whether the organisms can use sucrose, the organism must produce acid. The change in colour from phenol red to yellow indicate the acid production. When the colour change from phenol red to yellow that is referred as positive test of sucrose fermentation. When colour changed to pink or did not changed at all, that is referred as negative test of sucrose fermentation. The production of gas by the organisms is showed by the presence of air bubble in the tube. If there is no air bubble then no gas is produced. The phenol red sucrose broth is utilized to identify if the microorganism can use the sucrose for energy and carbon. RESULTS …show more content…
The tube of sucrose fermentation that was inoculated with culture of S.aureus changed the colour from phenol red to yellow and there was no air bubble in the tube. The tube of sucrose that was inoculated with both cultures, E.coli and S.aureus also turned from phenol red colour to yellow. There was an air bubble in this tube where cultures were in