Introduction The production of identical copies is called cloning, for example in identical twins they are clones where single embryos separate to become two and every single bit of their DNA is identical. So gene cloning means production of many identical copies of the same gene. Gene cloning requires a vector which introduces rDNA into the host cell and enzymes to introduce foreign DNA into vector DNA. Vector is plasmids and enzymes are restriction and ligase enzymes. Of course gene cloning has many research purposes, we can cover the cloned gene or protein products and also human can be treated with gene therapy. So this essay will be about the molecular cloning in the past and how it developed and what are the possibilities …show more content…
This research may achieve to alleviate the desperate shortage of human tissues for transplantation.
The differentiation process may control at least some cancer cells: Other gain from cloning is inducing cancer cells to differentiate. Because many types of cancer cells are less specialized than their normal counterparts, so precursors of cancer cells could be stem cells that fail to complete differentiation and then by using information gained from nuclear transfer technology we may be able to stop making tumors by induce the cells to mature.
Conclusion
In conclude, in the last 40 years molecular cloning has progressed from isolating and piecing two pieces of DNA together then screening of potential clones to assembling up to 10 DNA fragments with remarkable efficiency in few hours to designing DNA molecules and synthesizing them in vitro. All of these technologies give molecular biologists a powerful toolbox for exploring, manipulating and harnessing DNA so that make more possibilities to develop safer recombinant proteins for the treatment of diseases, quicker validation and release of new vaccines and also enhancement of gene