Sequencing Dna Synthesis Lab Report

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The first method of sequencing DNA was developed in the 1970’s by Fred Sanger; this came to be known as Sanger Sequencing (5). This method uses naturally occurring DNA synthesis, which proceeds from the 5’ phosphorus end to the 3’ hydroxide end. Synthesis requires a DNA template strand, DNA primer, nucleotides (dNTPs) and DNA polymerase. The trick behind this type of sequencing is to be able to stop the process at specific letters. Sanger was able to do this by adding modified chain terminators (ddNTPs), which lack the 3’OH group; therefore blocking DNA synthesis. This process results in varying lengths of DNAs, which are all smaller than the full length. Additionally, all of these small DNA pieces end with the same letter. This enabled Sanger

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