The first time the unknown bacteria was isolated, one of the colonies was observed to be bright yellow. This was important because it gave a clue as to which one was gram positive because looking for a yellow color was part of the gram positive dichotomous key. However, this conclusion that the yellow colony was positive and the white colony was negative was not certain until the conduction of the second gram stain. Also, even though Micrococcus was the only possible bacteria with colonies that have
Introduction: This experiment was performed to identify the unknown Species given, using selective and differential media. Materials and Methods: To begin this experiment, The Gram stain procedure will help identify a Gram-positive or Gram-negative bacteria. It begins with grabbing a clean glass slide and placing it on the glass staining rack. Flaming the inoculating loop sterile, use the DI water to create a bubble of DI water in the loop and placing it the center of the glass slide. Then flaming
Identification of bacteria within Unknown Culture #21 In this experiment, an unknown culture of two different types of bacteria was assigned to each person, a number of tests were performed to isolate and identify these bacterial cells. Based on knowledge from the previous experiments completed in lab, a basic understanding of each type of bacteria was used to create a flow chart that would aid the process of identifying the unknown bacteria within the culture. A gram stain that is performed initially
The bacterium was determined to be Gram- negative rods. The first attempt of gram stain the unknown appeared to look positive and cocci. But, the next day onwards for 4 consecutive days the unknown showed up as a gram- negative gram stain and appeared pink rods. Triple Sugar Iron Agar is a differential medium. It differentiates Gram-negative enteric bacteria based on the ability to reduce sulfur and ferment carbohydrates (lactose, sucrose, and glucose). This is indicated by the formation of acid
and most important test that should be performed on the bacteria is the Gram Stain. This test is a process of using multiple stains to differentiate between Gram Negative and Gram Positive organisms (Microbugz). If a bacterium is Gram Positive the cells will appear purple
Discussion and Conclusion The Gram positive Unk. A-DB was identified to be B. Cereus. This inference was reached though quite a bit of study. Firstly the Gram positive was found to be Bacilli shaped this eliminated the gram negative bacteia and three of the nine possible bacteria, leaveing B. cereus, B. megaterium, B. subtilis, C. diphtheriae, M. phlei and M. smegmatis. A timperature test was proformed so as to eliminate the two bacteria whose optimale temperature is 30°(alt+0176) C or exclude
The next thing I did after this was the gram stain. The purpose of this was to find my bug’s gram designation and morphology so that I would be working in the right direction. My bug retained the safranin stain which meant it was gram negative. The cells of the bacteria were rod shaped, therefore, their morphology was bacillus. The following test I did was the Oxidase test. The unknown tested negative for this particular test because the oxistrip failed to turn blue shortly
instead it sloughs off when rinsed with the ethanol alcohol. This leaves the color unretained and the counterstain safranin is absorbed, staining the bacteria pink. Once the slide was ready, we were able to observe the bacterial morphology under the microscope. We identified the bacterial shape as bacillus under 100X magnification with oil immersion. Figure 1: (Pseudomonas Aeruginosa mixture, Under 100X oil immersion) Oxidase Test: To test for the
stain is a differential stain where a decolorization step occurs due to two different basic stains. The Gram stain use a primary stain of crystal violet and secondary stain as safranin. The Gram stain tests for two large groups bacteria based on the cell walls. The Gram stain tells if the bacteria is either Gram positive (Violet) or Gram negative (Red). Purpose- The purpose of this lab was to
with subcultures made when needed and antiseptic techniques used throughout. How bacteria differ from each other chemically as well as their shape can be determined by a differential stain known as a Gram stain. Gram positive bacteria have thick cell walls made of many layers of peptidoglycan which enable the bacteria to retain the crystal violet stain. In contrast, gram negative bacteria have less peptidoglycan layers and contain porins in their outer membrane. For these reasons, gram negative
Bacterial Vaginosis What is Bacterial Vaginosis? Bacterial Vaginosis (BV) is the most common vaginal infection in women of childbearing age and it is caused by an overgrowth of bacteria in the vagina. How does BV occur? Normally, there is a certain amount of bacteria in the vagina that is considered healthy bacteria called Lactobacilli. This kind of bacteria makes the vagina slightly acidic and keeps harmful bacteria from growing. BV occurs when there is an imbalance and there are more harmful
Aims/Hypothesis(es) to be tested Bacterial Vaginosis (BV) is the most common cause of vaginal symptoms among women of reproductive age, ages 15-44 years. The number of lactobacilli in the vagina of women with BV is significantly lower than that in healthy women. While some women may be asymptomatic, most experience thin, white or yellow abnormal vaginal discharge and malodor, especially after intercourse. Women with BV have an increased risk of many gynecological complications. Bacterial infections have been
Germ theory of disease is one of the theories in the biology field. Germ theory of disease supports that microorganisms that are germs are the cause of diseases. This theory was first proposed by Girolamo Fracastoro in the 16th century but he was not able to provide any evidence to strengthen his proposal. This theory was not accepted easily by the citizen between the 16th century to the 18th century. The pre-existence of miasma theory was the obstacle to the acceptance of germ theory during that
Today, millions of women can implement their rights to vote in all elections in the united states of America, but this (rights) did not come easily to those women who sacrifice their lives to make this happen. In the speech “Address to Congress on Women’s Suffrage”, Catt delivered her message for women’s right from a firsthand account of what she had experienced as a woman living in the United States of America in the 19th century. She advocated for the rights of women to vote because she believes
Introduction It was near the end of 18th century that the geographical map of the world was fully navigated as a result of European exploration that initiated a series of changes to the global system today. The exploration started in the early 15th century with the Portuguese discoveries of Atlantic archipelagos and Africa, all the way to the discovery of America by Christopher Columbus in 1492, followed by the major exploration of the various parts of the world by European explorers. To the European
divided into two types, depending on the presence of nuclei inside cells. Eukaryotic cells have a well-defined nucleus surrounded by a nuclear envelope, whereas prokaryotic cells lack this compartment. All bacteria are prokaryotes. Bearze (2015) provided information about the Bacterial Cell Structure and Function. Vibrio cholerae is a gram negative, non-spore forming
Operational Reporting What is Operational Reporting? Operations management is a branch of management that involve the procedures of producing and redesigning of goods and services. Operational reporting is a reporting procedure about the operational details that present the team’s current activity. It aims to support the daily activities of the organization. Who do Operational Reporting? Business teams do operational reporting, including the members of the team. Even business leaders can do operational
I will explain, to you what is an organization. An organization is a social structure for a business, society, and association. It is also made to be a management structure of many different activities and responsibilities (Organization, 2017). Why do organizations need strategies? For one it is important. When planning you are always asking questions and having missing gaps. Having aa outline for a business, or a company, you will always need to plan for it to be successful Planning is my nature
The Structure Audit Sensing the need of when to take on responsibility has always been a strength of mine. I am a person who likes to have a plan to see that tasks are finished. That plan will include a way to get the job done with plenty of time to spare. I do not enjoy work piling up and trying to complete a responsibility at the last moment. I know how to put a plan in place and delegate it out so that it is accomplished. When an organization has a combination of a centralized and a decentralized
needs a certain structure to keep things running smoothly. The structure outlines how the group works, how members are accepted, who holds leadership positions, and how decisions are made. It also helps give the group a sense of identity and order and gives members a set of guidelines to follow. The structure is an essential part of any organization, so it is important for companies to choose the one that fits the group's goals and members (Chapter 9. Developing an Organizational Structure for the Initiative